3H3D

Drosophila Pumilio RNA binding domain (Puf domain)

Summary for 3H3D

• Entry DOI: 10.2210/pdb3h3d/pdb
• Descriptor: Maternal protein pumilio (2 entities in total)
• Functional Keywords: pumilio, puf, rna binding domain, alternative splicing, cytoplasm, developmental protein, phosphoprotein, repressor, rna-binding, translation regulation, rna binding protein
• Biological source: Drosophila melanogaster (Fruit fly)
• Total number of polymer chains: 2
• Total formula weight: 74022.80

Authors

Edwards, T.A.,Aggarwal, A.K.,Wharton, R.P. (deposition date: 2009-04-16, release date: 2009-04-28, Last modification date: 2024-03-20)

Primary citation

Edwards, T.A.,Pyle, S.E.,Wharton, R.P.,Aggarwal, A.K.
Structure of Pumilio reveals similarity between RNA and peptide binding motifs.
Cell(Cambridge,Mass.), 105:281-289, 2001

PubMed Abstract: Translation regulation plays an essential role in the differentiation and development of animal cells. One well-studied case is the control of hunchback mRNA during early Drosophila embryogenesis by the trans-acting factors Pumilio, Nanos, and Brain Tumor. We report here a crystal structure of the critical region of Pumilio, the Puf domain, that organizes a multivalent repression complex on the 3' untranslated region of hunchback mRNA. The structure reveals an extended, rainbow shaped molecule, with tandem helical repeats that bear unexpected resemblance to the armadillo repeats in beta-catenin and the HEAT repeats in protein phosphatase 2A. Based on the structure and genetic experiments, we identify putative interaction surfaces for hunchback mRNA and the cofactors Nanos and Brain Tumor. This analysis suggests that similar features in helical repeat proteins are used to bind extended peptides and RNA.

PubMed: 11336677
DOI: 10.1016/s0092-8674(01)00318-x

Experimental method

X-RAY DIFFRACTION (2.3 Å)