3H0G

RNA Polymerase II from Schizosaccharomyces pombe

Summary for 3H0G

• Entry DOI: 10.2210/pdb3h0g/pdb
• Descriptor: DNA-directed RNA polymerase II subunit rpb1, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerase II subunit RPB11, ... (14 entities in total)
• Functional Keywords: transcription, multi-protein complex, polymerase, dna-binding, dna-directed rna polymerase, magnesium, metal-binding, nucleotidyltransferase, nucleus, phosphoprotein, transferase, zinc-finger
• Biological source: Schizosaccharomyces pombe; More
• Cellular location: Nucleus : P36594 O13877 P87123 P48011 Q02061 P37382 O74825 Q09191 P36595 O14459 Q92399; Nucleus, nucleolus : O74635
• Total number of polymer chains: 24
• Total formula weight: 996102.68

Authors

Spahr, H.,Calero, G.,Bushnell, D.A.,Kornberg, R.D. (deposition date: 2009-04-09, release date: 2009-08-25, Last modification date: 2023-09-06)

Primary citation

Spahr, H.,Calero, G.,Bushnell, D.A.,Kornberg, R.D.
Schizosacharomyces pombe RNA polymerase II at 3.6-A resolution.
Proc.Natl.Acad.Sci.USA, 106:9185-9190, 2009

PubMed Abstract: The second structure of a eukaryotic RNA polymerase II so far determined, that of the enzyme from the fission yeast Schizosaccharomyces pombe, is reported here. Comparison with the previous structure of the enzyme from the budding yeast Saccharomyces cerevisiae reveals differences in regions implicated in start site selection and transcription factor interaction. These aspects of the transcription mechanism differ between S. pombe and S. cerevisiae, but are conserved between S. pombe and humans. Amino acid changes apparently responsible for the structural differences are also conserved between S. pombe and humans, suggesting that the S. pombe structure may be a good surrogate for that of the human enzyme.

PubMed: 19458260
DOI: 10.1073/pnas.0903361106

Experimental method

X-RAY DIFFRACTION (3.65 Å)