3H0G

RNA Polymerase II from Schizosaccharomyces pombe

3H0G の概要

• エントリーDOI: 10.2210/pdb3h0g/pdb
• 分子名称: DNA-directed RNA polymerase II subunit rpb1, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerase II subunit RPB11, ... (14 entities in total)
• 機能のキーワード: transcription, multi-protein complex, polymerase, dna-binding, dna-directed rna polymerase, magnesium, metal-binding, nucleotidyltransferase, nucleus, phosphoprotein, transferase, zinc-finger
• 由来する生物種: Schizosaccharomyces pombe; 詳細
• 細胞内の位置: Nucleus : P36594 O13877 P87123 P48011 Q02061 P37382 O74825 Q09191 P36595 O14459 Q92399; Nucleus, nucleolus : O74635
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 996102.68

構造登録者

Spahr, H.,Calero, G.,Bushnell, D.A.,Kornberg, R.D. (登録日: 2009-04-09, 公開日: 2009-08-25, 最終更新日: 2024-11-06)

主引用文献

Spahr, H.,Calero, G.,Bushnell, D.A.,Kornberg, R.D.
Schizosacharomyces pombe RNA polymerase II at 3.6-A resolution.
Proc.Natl.Acad.Sci.USA, 106:9185-9190, 2009

PubMed Abstract: The second structure of a eukaryotic RNA polymerase II so far determined, that of the enzyme from the fission yeast Schizosaccharomyces pombe, is reported here. Comparison with the previous structure of the enzyme from the budding yeast Saccharomyces cerevisiae reveals differences in regions implicated in start site selection and transcription factor interaction. These aspects of the transcription mechanism differ between S. pombe and S. cerevisiae, but are conserved between S. pombe and humans. Amino acid changes apparently responsible for the structural differences are also conserved between S. pombe and humans, suggesting that the S. pombe structure may be a good surrogate for that of the human enzyme.

PubMed: 19458260
DOI: 10.1073/pnas.0903361106

実験手法

X-RAY DIFFRACTION (3.65 Å)