3H0D

Crystal structure of CtsR in complex with a 26bp DNA duplex

3H0D の概要

• エントリーDOI: 10.2210/pdb3h0d/pdb
• 分子名称: CtsR, DNA (26-MER), PHOSPHATE ION, ... (5 entities in total)
• 機能のキーワード: protein dna complex, winged hth domain, 4-helix bundle, dna tandem repeat, transcription-dna complex, transcription/dna
• 由来する生物種: Bacillus stearothermophilus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 53250.07

構造登録者

Clausen, T.,Fuhrmann, J. (登録日: 2009-04-09, 公開日: 2009-06-30, 最終更新日: 2024-11-20)

主引用文献

Fuhrmann, J.,Schmidt, A.,Spiess, S.,Lehner, A.,Turgay, K.,Mechtler, K.,Charpentier, E.,Clausen, T.
McsB is a protein arginine kinase that phosphorylates and inhibits the heat-shock regulator CtsR
Science, 324:1323-1327, 2009

PubMed Abstract: All living organisms face a variety of environmental stresses that cause the misfolding and aggregation of proteins. To eliminate damaged proteins, cells developed highly efficient stress response and protein quality control systems. We performed a biochemical and structural analysis of the bacterial CtsR/McsB stress response. The crystal structure of the CtsR repressor, in complex with DNA, pinpointed key residues important for high-affinity binding to the promoter regions of heat-shock genes. Moreover, biochemical characterization of McsB revealed that McsB specifically phosphorylates arginine residues in the DNA binding domain of CtsR, thereby impairing its function as a repressor of stress response genes. Identification of the CtsR/McsB arginine phospho-switch expands the repertoire of possible protein modifications involved in prokaryotic and eukaryotic transcriptional regulation.

PubMed: 19498169
DOI: 10.1126/science.1170088

実験手法

X-RAY DIFFRACTION (2.4 Å)