3GVT
Structure and RNA binding of the mouse Pumilio-2 Puf Domain
Summary for 3GVT
| Entry DOI | 10.2210/pdb3gvt/pdb |
| Related | 3GVO |
| Descriptor | Pumilio homolog 2, 2,3-DIHYDROXY-1,4-DITHIOBUTANE, GLYCEROL, ... (4 entities in total) |
| Functional Keywords | rna-binding, pumilio, puf domain, rna binding protein |
| Biological source | Mus musculus (mouse) |
| Cellular location | Cytoplasm (Probable): Q80U58 |
| Total number of polymer chains | 2 |
| Total formula weight | 81243.54 |
| Authors | Jenkins, H.T.,Edwards, T.A. (deposition date: 2009-03-31, release date: 2009-06-30, Last modification date: 2023-11-01) |
| Primary citation | Jenkins, H.T.,Baker-Wilding, R.,Edwards, T.A. Structure and RNA binding of the mouse Pumilio-2 Puf domain J.Struct.Biol., 167:271-276, 2009 Cited by PubMed Abstract: Puf proteins control translation through the interaction of a C-terminal Puf domain with specific sequences present in the 3' untranslated region of messenger RNAs. In Drosophila, binding of the protein Pumilio to mRNA leads to translational repression which is required for anterior/posterior patterning during embryogenesis. The vertebrate Pumilio homologue 2 (Pum2) has been implicated in controlling germ cell development through interactions with the RNA binding proteins deleted in azoospermia (DAZ), DAZ-like (DAZL) and BOULE. We present the 1.6A resolution X-ray crystal structure of the Puf domain from murine Pum2 and demonstrate that this domain is capable of binding with nanomolar affinity to RNA sequences from the hunchback Nanos response element (NRE) and a previously identified Pum2 binding element (PBE). PubMed: 19540345DOI: 10.1016/j.jsb.2009.06.007 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.8 Å) |
Structure validation
Download full validation report
