3GVT

Structure and RNA binding of the mouse Pumilio-2 Puf Domain

3GVT の概要

• エントリーDOI: 10.2210/pdb3gvt/pdb
• 関連するPDBエントリー: 3GVO
• 分子名称: Pumilio homolog 2, 2,3-DIHYDROXY-1,4-DITHIOBUTANE, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: rna-binding, pumilio, puf domain, rna binding protein
• 由来する生物種: Mus musculus (mouse)
• 細胞内の位置: Cytoplasm (Probable): Q80U58
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 81243.54

構造登録者

Jenkins, H.T.,Edwards, T.A. (登録日: 2009-03-31, 公開日: 2009-06-30, 最終更新日: 2023-11-01)

主引用文献

Jenkins, H.T.,Baker-Wilding, R.,Edwards, T.A.
Structure and RNA binding of the mouse Pumilio-2 Puf domain
J.Struct.Biol., 167:271-276, 2009

PubMed Abstract: Puf proteins control translation through the interaction of a C-terminal Puf domain with specific sequences present in the 3' untranslated region of messenger RNAs. In Drosophila, binding of the protein Pumilio to mRNA leads to translational repression which is required for anterior/posterior patterning during embryogenesis. The vertebrate Pumilio homologue 2 (Pum2) has been implicated in controlling germ cell development through interactions with the RNA binding proteins deleted in azoospermia (DAZ), DAZ-like (DAZL) and BOULE. We present the 1.6A resolution X-ray crystal structure of the Puf domain from murine Pum2 and demonstrate that this domain is capable of binding with nanomolar affinity to RNA sequences from the hunchback Nanos response element (NRE) and a previously identified Pum2 binding element (PBE).

PubMed: 19540345
DOI: 10.1016/j.jsb.2009.06.007

実験手法

X-RAY DIFFRACTION (2.8 Å)