3GRV

Crystal Structure of the Complex between Adenosine and Methanocaldococcus jannaschi Dim1

3GRV の概要

• エントリーDOI: 10.2210/pdb3grv/pdb
• 関連するPDBエントリー: 1QYR 1ZQ9 2H1R 3FUT 3FUU 3FUV 3FUW 3FUX 3FYC 3FYD 3GRR 3GRU 3GRY
• 分子名称: Dimethyladenosine transferase, ADENOSINE, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: dimethyladenosine transferase, rossmann fold, ribosomal assembly s-adenosyl-l-methionine, rrna, methyltransferase, rna-binding, rrna processing, s-adenosyl-l-methionine, transferase
• 由来する生物種: Methanocaldococcus jannaschii (Methanococcus jannaschii)
• 細胞内の位置: Cytoplasm : Q58435
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34336.67

構造登録者

Scarsdale, J.N.,Musayev, F.N.,Rife, J.P. (登録日: 2009-03-26, 公開日: 2010-03-31, 最終更新日: 2023-09-06)

主引用文献

O'Farrell, H.C.,Musayev, F.N.,Scarsdale, J.N.,Rife, J.P.
Binding of adenosine-based ligands to the MjDim1 rRNA methyltransferase: implications for reaction mechanism and drug design.
Biochemistry, 49:2697-2704, 2010

PubMed Abstract: The KsgA/Dim1 family of proteins is intimately involved in ribosome biogenesis in all organisms. These enzymes share the common function of dimethylating two adenosine residues near the 3'-OH end of the small subunit rRNA; orthologs in the three kingdoms, along with eukaryotic organelles, have evolved additional functions in rRNA processing, ribosome assembly, and, surprisingly, transcription in mitochondria. The methyltransferase reaction is intriguingly elaborate. The enzymes can bind to naked small subunit rRNA but cannot methylate their target bases until a subset of ribosomal proteins have bound and the nascent subunit has reached a certain level of maturity. Once this threshold is reached, the enzyme must stabilize two adenosines into the active site at separate times and two methyl groups must be transferred to each adenosine, with concomitant exchanges of the product S-adenosyl-l-homocysteine and the methyl donor substrate S-adenosyl-l-methionine. A detailed molecular understanding of this mechanism is currently lacking. Structural analysis of the interactions between the enzyme and substrate will aid in this understanding. Here we present the structure of KsgA from Methanocaldococcus jannaschii in complex with several ligands, including the first structure of S-adenosyl-l-methionine bound to a KsgA/Dim1 enzyme in a catalytically productive way. We also discuss the inability thus far to determine a structure of a target adenosine bound in its active site.

PubMed: 20163168
DOI: 10.1021/bi901875x

実験手法

X-RAY DIFFRACTION (1.9 Å)