3GOE

Molecular Mimicry of SUMO promotes DNA repair

3GOE の概要

• エントリーDOI: 10.2210/pdb3goe/pdb
• 分子名称: DNA repair protein rad60, CALCIUM ION (3 entities in total)
• 機能のキーワード: sumo-like domain, dna repair, sumoylation, sumo, genome stability, dna damage, dna recombination, nucleus, phosphoprotein, recombination, replication
• 由来する生物種: Schizosaccharomyces pombe (Fission yeast)
• 細胞内の位置: Nucleus: Q9USX3
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9833.13

構造登録者

Perry, J.J.P. (登録日: 2009-03-19, 公開日: 2009-04-14, 最終更新日: 2023-09-06)

主引用文献

Prudden, J.,Perry, J.J.,Arvai, A.S.,Tainer, J.A.,Boddy, M.N.
Molecular mimicry of SUMO promotes DNA repair.
Nat.Struct.Mol.Biol., 16:509-516, 2009

PubMed Abstract: Rad60 family members contain functionally enigmatic, integral SUMO-like domains (SLDs). We show here that despite their divergence from SUMO, each Rad60 SLD interacts with a subset of SUMO pathway enzymes: SLD2 specifically binds the SUMO E2 conjugating enzyme (Ubc9), whereas SLD1 binds the SUMO E1 (Fub2, also called Uba2) activating and E3 (Pli1, also called Siz1 and Siz2) specificity enzymes. The molecular basis of this selectivity is revealed by our 0.97-A resolution crystal structure of Rad60 SLD2, which shows that apart from the conserved non-substrate SUMO:Ubc9 interface, the surface features of SLD2 are distinct from those of SUMO. Abrogation of the SLD2:Ubc9 FEG motif-dependent interaction results in hypersensitivity to genotoxic stress and an increase in spontaneous recombination associated with aberrant replication forks. Our results provide a mechanistic basis for the near-synonymous roles of Rad60 and SUMO in survival of genotoxic stress and suggest unprecedented DNA-damage-response functions for SLDs in regulating sumoylation.

PubMed: 19363481
DOI: 10.1038/nsmb.1582

実験手法

X-RAY DIFFRACTION (0.97 Å)