3GGW

Crystal Structure of FAB F22-4 in complex with a Carbohydrate-mimetic peptide

3GGW の概要

• エントリーDOI: 10.2210/pdb3ggw/pdb
• 関連するPDBエントリー: 3BZ4 3C5S 3C6S
• 分子名称: FAB F22-4 LIGHT CHAIN, FAB F22-4 HEAVY CHAIN, PEPTIDE B1, ... (4 entities in total)
• 機能のキーワード: peptide-carbohydrate mimicry, lps, shigella flexneri, antibody complex, immune system
• 由来する生物種: MUS MUSCULUS (MOUSE); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 98784.32

構造登録者

Saul, F.A.,Vulliez-Le Normand, B.,Bentley, G.A. (登録日: 2009-03-02, 公開日: 2009-03-31, 最終更新日: 2024-11-13)

主引用文献

Theillet, F.-X.,Saul, F.A.,Vulliez-Le Normand, B.,Hoos, S.,Felici, F.,Weintraub, A.,Mulard, L.A.,Phalipon, A.,Delepierre, M.,Bentley, G.A.
Structural Mimicry of O-Antigen by a Peptide Revealed in a Complex with an Antibody Raised against Shigella flexneri Serotype 2a
J.Mol.Biol., 388:839-850, 2009

PubMed Abstract: The use of carbohydrate-mimicking peptides to induce immune responses against surface polysaccharides of pathogenic bacteria offers a novel approach to vaccine development. Factors governing antigenic and immunogenic mimicry, however, are complex and poorly understood. We have addressed this question using the anti-lipopolysaccharide monoclonal antibody F22-4, which was raised against Shigella flexneri serotype 2a and shown to protect against homologous infection in a mouse model. In a previous crystallographic study, we described F22-4 in complex with two synthetic fragments of the O-antigen, the serotype-specific saccharide moiety of lipopolysaccharide. Here, we present a crystallographic and NMR study of the interaction of F22-4 with a dodecapeptide selected by phage display using the monoclonal antibody. Like the synthetic decasaccharide, the peptide binds to F22-4 with micromolar affinity. Although the peptide and decasaccharide use very similar regions of the antigen-binding site, indicating good antigenic mimicry, immunogenic mimicry by the peptide was not observed. The F22-4-antigen interaction is significantly more hydrophobic with the peptide than with oligosaccharides; nonetheless, all hydrogen bonds formed between the peptide and F22-4 have equivalents in the oligosaccharide complex. Two bridging water molecules are also in common, adding to partial structural mimicry. Whereas the bound peptide is entirely helical, its structure in solution, as shown by NMR, is helical in the central region only. Moreover, docking the NMR structure into the antigen-binding site shows that steric hindrance would occur, revealing poor complementarity between the major solution conformation and the antibody that could contribute to the absence of immunogenic mimicry.

PubMed: 19328810
DOI: 10.1016/j.jmb.2009.03.057

実験手法

X-RAY DIFFRACTION (1.7 Å)