3GFK

Crystal structure of Bacillus subtilis Spx/RNA polymerase alpha subunit C-terminal domain complex

3GFK の概要

• エントリーDOI: 10.2210/pdb3gfk/pdb
• 分子名称: Regulatory protein spx, DNA-directed RNA polymerase subunit alpha (3 entities in total)
• 機能のキーワード: protein-protein complex, cytoplasm, redox-active center, stress response, transcription, transcription regulation, dna-directed rna polymerase, nucleotidyltransferase, transferase, transcription-transferase complex, transcription/transferase
• 由来する生物種: Bacillus subtilis; 詳細
• 細胞内の位置: Cytoplasm (Probable): O31602
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 24670.36

構造登録者

Lamour, V.,Westblade, L.F.,Campbell, E.A.,Darst, S.A. (登録日: 2009-02-27, 公開日: 2009-03-10, 最終更新日: 2024-10-30)

主引用文献

Lamour, V.,Westblade, L.F.,Campbell, E.A.,Darst, S.A.
Crystal structure of the in vivo-assembled Bacillus subtilis Spx/RNA polymerase alpha subunit C-terminal domain complex
J.Struct.Biol., 168:352-356, 2009

PubMed Abstract: The Bacillus subtilis Spx protein is a global transcription factor that interacts with the C-terminal domain of the RNA polymerase alpha subunit (alphaCTD) and regulates transcription of genes involved in thiol-oxidative stress, sporulation, competence, and organosulfur metabolism. Here we determined the X-ray crystal structure of the Spx/alphaCTD complex from an entirely new crystal form than previously reported [Newberry, K.J., Nakano, S., Zuber, P., Brennan, R.G., 2005. Crystal structure of the Bacillus subtilis anti-alpha, global transcriptional regulator, Spx, in complex with the alpha C-terminal domain of RNA polymerase. Proc. Natl. Acad. Sci. USA 102, 15839-15844]. Comparison of the previously reported sulfate-bound complex and our sulfate-free complex reveals subtle conformational changes that may be important for the role of Spx in regulating organosulfur metabolism.

PubMed: 19580872
DOI: 10.1016/j.jsb.2009.07.001

実験手法

X-RAY DIFFRACTION (2.3 Å)