3GDP

Hydroxynitrile lyase from almond, monoclinic crystal form

3GDP の概要

• エントリーDOI: 10.2210/pdb3gdp/pdb
• 関連するPDBエントリー: 1ju2 3gdn
• 分子名称: R-oxynitrile lyase isoenzyme 1, alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-L-fucopyranose-(1-3)]2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-L-fucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (10 entities in total)
• 機能のキーワード: hydroxynitrile lyase, flavin, gmc oxidoreductase, almond, cyanogenesis, flavoprotein, lyase
• 由来する生物種: Prunus dulcis (sweet almond)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 118756.56

構造登録者

Dreveny, I.,Gruber, K.,Kratky, C. (登録日: 2009-02-24, 公開日: 2009-03-24, 最終更新日: 2024-11-20)

主引用文献

Dreveny, I.,Andryushkova, A.S.,Glieder, A.,Gruber, K.,Kratky, C.
Substrate binding in the FAD-dependent hydroxynitrile lyase from almond provides insight into the mechanism of cyanohydrin formation and explains the absence of dehydrogenation activity.
Biochemistry, 48:3370-3377, 2009

PubMed Abstract: In a large number of plant species hydroxynitrile lyases catalyze the decomposition of cyanohydrins in order to generate hydrogen cyanide upon tissue damage. Hydrogen cyanide serves as a deterrent against herbivores and fungi. In vitro hydroxynitrile lyases are proficient biocatalysts for the stereospecific synthesis of cyanohydrins. Curiously, hydroxynitrile lyases from different species are completely unrelated in structure and substrate specificity despite catalyzing the same reaction. The hydroxynitrile lyase from almond shows close resemblance to flavoproteins of the glucose-methanol-choline oxidoreductase family. We report here 3D structural data of this lyase with the reaction product benzaldehyde bound within the active site, which allow unambiguous assignment of the location of substrate binding. Based on the binding geometry, a reaction mechanism is proposed that involves one of the two conserved active site histidine residues acting as a general base abstracting the proton from the cyanohydrin hydroxyl group. Site-directed mutagenesis shows that both active site histidines are required for the reaction to occur. There is no evidence that the flavin cofactor directly participates in the reaction. Comparison with other hydroxynitrile lyases reveals a large diversity of active site architectures, which, however, share the common features of a general active site base and a nearby patch with positive electrostatic potential. On the basis of the difference in substrate binding geometry between the FAD-dependent HNL from almond and the related oxidases, we can rationalize why the HNL does not act as an oxidase.

PubMed: 19256550
DOI: 10.1021/bi802162s

実験手法

X-RAY DIFFRACTION (1.57 Å)