3GCX

PCSK9:EGFA (pH 7.4)

3GCX の概要

• エントリーDOI: 10.2210/pdb3gcx/pdb
• 関連するPDBエントリー: 3GCW
• 分子名称: Proprotein convertase subtilisin/kexin type 9, Low-density lipoprotein receptor, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: pcsk9, ldl receptor, autocatalytic cleavage, cholesterol metabolism, disease mutation, glycoprotein, hydrolase, lipid metabolism, phosphoprotein, protease, secreted, serine protease, steroid metabolism, zymogen, coated pit, egf-like domain, endocytosis, host-virus interaction, ldl, lipid transport, membrane, receptor, transmembrane, transport, protein binding
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Secreted: Q8NBP7 Q8NBP7; Cell membrane; Single-pass type I membrane protein: P01130
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 77857.85

構造登録者

McNutt, M.C.,Kwon, H.J.,Chen, C.,Chen, J.R.,Horton, J.D.,Lagace, T.A. (登録日: 2009-02-22, 公開日: 2009-03-03, 最終更新日: 2024-11-06)

主引用文献

McNutt, M.C.,Kwon, H.J.,Chen, C.,Chen, J.R.,Horton, J.D.,Lagace, T.A.
Antagonism of Secreted PCSK9 Increases Low Density Lipoprotein Receptor Expression in HepG2 Cells.
J.Biol.Chem., 284:10561-10570, 2009

PubMed Abstract: PCSK9 is a secreted protein that degrades low density lipoprotein receptors (LDLRs) in liver by binding to the epidermal growth factor-like repeat A (EGF-A) domain of the LDLR. It is not known whether PCSK9 causes degradation of LDLRs within the secretory pathway or following secretion and reuptake via endocytosis. Here we show that a mutation in the LDLR EGF-A domain associated with familial hypercholesterolemia, H306Y, results in increased sensitivity to exogenous PCSK9-mediated cellular degradation because of enhanced PCSK9 binding affinity. The crystal structure of the PCSK9-EGF-A(H306Y) complex shows that Tyr-306 forms a hydrogen bond with Asp-374 in PCSK9 at neutral pH, which strengthens the interaction with PCSK9. To block secreted PCSK9 activity, LDLR (H306Y) subfragments were added to the medium of HepG2 cells stably overexpressing wild-type PCSK9 or gain-of-function PCSK9 mutants associated with hypercholesterolemia (D374Y or S127R). These subfragments blocked secreted PCSK9 binding to cell surface LDLRs and resulted in the recovery of LDLR levels to those of control cells. We conclude that PCSK9 acts primarily as a secreted factor to cause LDLR degradation. These studies support the concept that pharmacological inhibition of the PCSK9-LDLR interaction extracellularly will increase hepatic LDLR expression and lower plasma low density lipoprotein levels.

PubMed: 19224862
DOI: 10.1074/jbc.M808802200

実験手法

X-RAY DIFFRACTION (2.7 Å)