3G5H

Crystallographic analysis of cytochrome P450 cyp121

3G5H の概要

• エントリーDOI: 10.2210/pdb3g5h/pdb
• 関連するPDBエントリー: 1N40 3G5F
• 分子名称: Cytochrome P450 121, PROTOPORPHYRIN IX CONTAINING FE, (3S,6S)-3,6-bis(4-hydroxybenzyl)piperazine-2,5-dione, ... (5 entities in total)
• 機能のキーワード: cytochrome p450, cyp121, tuberculosis, cyclodipeptide, substrate, oxidoreductase
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 44344.76

構造登録者

Belin, P.,Le Du, M.H.,Gondry, M. (登録日: 2009-02-05, 公開日: 2009-04-21, 最終更新日: 2024-03-20)

主引用文献

Belin, P.,Le Du, M.H.,Fielding, A.,Lequin, O.,Jacquet, M.,Charbonnier, J.B.,Lecoq, A.,Thai, R.,Courcon, M.,Masson, C.,Dugave, C.,Genet, R.,Pernodet, J.L.,Gondry, M.
Identification and structural basis of the reaction catalyzed by CYP121, an essential cytochrome P450 in Mycobacterium tuberculosis.
Proc.Natl.Acad.Sci.USA, 106:7426-7431, 2009

PubMed Abstract: The gene encoding the cytochrome P450 CYP121 is essential for Mycobacterium tuberculosis. However, the CYP121 catalytic activity remains unknown. Here, we show that the cyclodipeptide cyclo(l-Tyr-l-Tyr) (cYY) binds to CYP121, and is efficiently converted into a single major product in a CYP121 activity assay containing spinach ferredoxin and ferredoxin reductase. NMR spectroscopy analysis of the reaction product shows that CYP121 catalyzes the formation of an intramolecular C-C bond between 2 tyrosyl carbon atoms of cYY resulting in a novel chemical entity. The X-ray structure of cYY-bound CYP121, solved at high resolution (1.4 A), reveals one cYY molecule with full occupancy in the large active site cavity. One cYY tyrosyl approaches the heme and establishes a specific H-bonding network with Ser-237, Gln-385, Arg-386, and 3 water molecules, including the sixth iron ligand. These observations are consistent with low temperature EPR spectra of cYY-bound CYP121 showing a change in the heme environment with the persistence of the sixth heme iron ligand. As the carbon atoms involved in the final C-C coupling are located 5.4 A apart according to the CYP121-cYY complex crystal structure, we propose that C-C coupling is concomitant with substrate tyrosyl movements. This study provides insight into the catalytic activity, mechanism, and biological function of CYP121. Also, it provides clues for rational design of putative CYP121 substrate-based antimycobacterial agents.

PubMed: 19416919
DOI: 10.1073/pnas.0812191106

実験手法

X-RAY DIFFRACTION (1.4 Å)