3FY1

The Acidic Mammalian Chitinase catalytic domain in complex with methylallosamidin

3FY1 の概要

• エントリーDOI: 10.2210/pdb3fy1/pdb
• 関連するPDBエントリー: 3FXY
• 分子名称: Acidic mammalian chitinase, 2-acetamido-2-deoxy-6-O-methyl-alpha-D-allopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-allopyranose, ALLOSAMIZOLINE, ... (4 entities in total)
• 機能のキーワード: chitinase, asthma, inhibitor, chitin degradation, methylallosamidin, alternative splicing, carbohydrate metabolism, chitin-binding, cytoplasm, glycosidase, hydrolase, polymorphism, polysaccharide degradation, secreted
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 89716.06

構造登録者

Olland, A.M. (登録日: 2009-01-21, 公開日: 2009-03-10, 最終更新日: 2024-11-06)

主引用文献

Olland, A.M.,Strand, J.,Presman, E.,Czerwinski, R.,Joseph-McCarthy, D.,Krykbaev, R.,Schlingmann, G.,Chopra, R.,Lin, L.,Fleming, M.,Kriz, R.,Stahl, M.,Somers, W.,Fitz, L.,Mosyak, L.
Triad of polar residues implicated in pH specificity of acidic mammalian chitinase.
Protein Sci., 18:569-578, 2009

PubMed Abstract: Acidic mammalian chitinase (AMCase) is a mammalian chitinase that has been implicated in allergic asthma. One of only two active mammalian chinases, AMCase, is distinguished from other chitinases by several unique features. Here, we present the novel structure of the AMCase catalytic domain, both in the apo form and in complex with the inhibitor methylallosamidin, determined to high resolution by X-ray crystallography. These results provide a structural basis for understanding some of the unique characteristics of this enzyme, including the low pH optimum and the preference for the beta-anomer of the substrate. A triad of polar residues in the second-shell is found to modulate the highly conserved chitinase active site. As a novel target for asthma therapy, structural details of AMCase activity will help guide the future design of specific and potent AMCase inhibitors.

PubMed: 19241384
DOI: 10.1002/pro.63

実験手法

X-RAY DIFFRACTION (1.7 Å)