3FX4
Porcine aldehyde reductase in ternary complex with inhibitor
Summary for 3FX4
| Entry DOI | 10.2210/pdb3fx4/pdb |
| Related | 2AO0 3CV7 |
| Descriptor | Alcohol dehydrogenase [NADP+], SULFATE ION, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, ... (5 entities in total) |
| Functional Keywords | tim barrel, aldo-keto reductase, ternary complex, acetylation, nadp, oxidoreductase |
| Biological source | Sus scrofa (Pig) |
| Total number of polymer chains | 1 |
| Total formula weight | 38025.88 |
| Authors | Carbone, V.,El-Kabbani, O. (deposition date: 2009-01-20, release date: 2009-12-22, Last modification date: 2023-11-01) |
| Primary citation | Carbone, V.,Giglio, M.,Chung, R.,Huyton, T.,Adams, J.,Maccari, R.,Ottana, R.,Hara, A.,El-Kabbani, O. Structure of aldehyde reductase in ternary complex with a 5-arylidene-2,4-thiazolidinedione aldose reductase inhibitor. Eur.J.Med.Chem., 45:1140-1145, 2010 Cited by PubMed Abstract: The structure of aldehyde reductase (ALR1) in ternary complex with the coenzyme NADPH and [5-(3-carboxymethoxy-4-methoxybenzylidene)-2,4-dioxothiazolidin-3-yl]acetic acid (CMD), a potent inhibitor of aldose reductase (ALR2), was determined at 1.99A resolution. The partially disordered inhibitor formed a tight network of hydrogen bonds with the active site residues (Tyr50 and His113) and coenzyme. Molecular modelling calculations and inhibitory activity measurements of CMD and [5-(3-hydroxy-4-methoxybenzylidene)-2,4-dioxothiazolidin-3-yl]acetic acid (HMD) indicated that pi-stacking interactions with several conserved active site tryptophan residues and hydrogen-bonding interactions with the non-conserved C-terminal residue Leu300 in ALR2 (Pro301 in ALR1) contributed to inhibitor selectivity. In particular for the potent inhibitor CMD, the rotameric state of the conserved residue Trp219 (Trp220 in ALR1) is important in forming a pi-stacking interaction with the inhibitor in ALR2 and contributes to the difference in the binding of the inhibitor to the enzymes. PubMed: 20036445DOI: 10.1016/j.ejmech.2009.12.019 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.99 Å) |
Structure validation
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