3FU2

Cocrystal structure of a class-I preQ1 riboswitch

3FU2 の概要

• エントリーDOI: 10.2210/pdb3fu2/pdb
• 関連するPDBエントリー: 3FU4
• 分子名称: PreQ1 riboswitch, 7-DEAZA-7-AMINOMETHYL-GUANINE, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: rna; aptamer; riboswitch; queuosine; preq1, rna
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 33404.81

構造登録者

Klein, D.J.,Edwards, T.E.,Ferre-D'Amare, A.R. (登録日: 2009-01-13, 公開日: 2009-03-03, 最終更新日: 2024-02-21)

主引用文献

Klein, D.J.,Edwards, T.E.,Ferre-D'Amare, A.R.
Cocrystal structure of a class I preQ1 riboswitch reveals a pseudoknot recognizing an essential hypermodified nucleobase
Nat.Struct.Mol.Biol., 16:343-344, 2009

PubMed Abstract: Riboswitches are mRNA domains that bind metabolites and modulate gene expression in cis. We report cocrystal structures of a remarkably compact riboswitch (34 nucleotides suffice for ligand recognition) from Bacillus subtilis that is selective for the essential nucleobase preQ(1) (7-aminomethyl-7-deazaguanine). The structures reveal a previously unrecognized pseudoknot fold and suggest a conserved gene-regulatory mechanism whereby ligand binding promotes sequestration of an RNA segment that otherwise assembles into a transcriptional antiterminator.

PubMed: 19234468
DOI: 10.1038/nsmb.1563

実験手法

X-RAY DIFFRACTION (2.85 Å)