3FPJ

Crystal Structure of E81Q mutant of MtNAS in complex with S-ADENOSYLMETHIONINE

Summary for 3FPJ

• Entry DOI: 10.2210/pdb3fpj/pdb
• Related: 3FPE 3FPF 3FPG 3FPH
• Descriptor: Putative uncharacterized protein, S-ADENOSYLMETHIONINE, BROMIDE ION, ... (5 entities in total)
• Functional Keywords: thermonicotianamine, nicotianamine, biosynthetic protein, transferase
• Biological source: Methanothermobacter thermautotrophicus
• Total number of polymer chains: 2
• Total formula weight: 69018.39

Authors

Dreyfus, C.,Pignol, D.,Arnoux, P. (deposition date: 2009-01-05, release date: 2009-10-06, Last modification date: 2024-10-30)

Primary citation

Dreyfus, C.,Lemaire, D.,Mari, S.,Pignol, D.,Arnoux, P.
Crystallographic snapshots of iterative substrate translocations during nicotianamine synthesis in Archaea
Proc.Natl.Acad.Sci.USA, 106:16180-16184, 2009

PubMed Abstract: Nicotianamine (NA), a small molecule ubiquitous in plants, is an important divalent metal chelator and the main precursor of phytosiderophores. Nicotianamine synthase (NAS) is the enzyme catalyzing NA synthesis by the condensation of three aminopropyl moieties of S-adenosylmethionine (SAM) and the cyclization of one of them to form an azetidine ring. Here we report five crystal structures of an archaeal NAS from Methanothermobacter thermautotrophicus, either free or in complex with its product(s) and substrate(s). These structures reveal a two-domains fold arrangement of MtNAS, a small molecule related to NA (named here thermoNicotianamine or tNA), and an original mechanism of synthesis in a buried reaction chamber. This reaction chamber is open to the solvent through a small inlet, and a single active site allows the selective entrance of only one substrate at a time that is then processed and translocated stepwise.

PubMed: 19805277
DOI: 10.1073/pnas.0904439106

Experimental method

X-RAY DIFFRACTION (1.8 Å)