3FPB

The Structure of Sarcoplasmic Reticulum Ca2+-ATPase Bound To Cyclopiazonic acid with ATP

3FPB の概要

• エントリーDOI: 10.2210/pdb3fpb/pdb
• 関連するPDBエントリー: 3FGO
• 分子名称: Sarcoplasmic/endoplasmic reticulum calcium ATPase 1, MAGNESIUM ION, TETRAFLUOROMAGNESATE(2-), ... (7 entities in total)
• 機能のキーワード: calcium pump, serca, nonhydrolyzable atp analog, p-type atpase, cyclopiazonic acid, ion transport, phosphoprotein, sarcoplasmic reticulum, transmembrane, hydrolase
• 由来する生物種: Oryctolagus cuniculus (Rabbit)
• 細胞内の位置: Endoplasmic reticulum membrane ; Multi-pass membrane protein : P04191
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 110634.15

構造登録者

Moncoq, K.,Morth, J.P.,Bublitz, M.,Laursen, M.,Nissen, P.,Young, H.S. (登録日: 2009-01-05, 公開日: 2009-04-07, 最終更新日: 2025-03-19)

主引用文献

Laursen, M.,Bublitz, M.,Moncoq, K.,Olesen, C.,Moller, J.V.,Young, H.S.,Nissen, P.,Morth, J.P.
Cyclopiazonic acid is complexed to a divalent metal ion when bound to the sarcoplasmic reticulum Ca2+-ATPase.
J.Biol.Chem., 284:13513-13518, 2009

PubMed Abstract: We have determined the structure of the sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA) in an E2.P(i)-like form stabilized as a complex with MgF(4)(2-), an ATP analog, adenosine 5'-(beta,gamma-methylene)triphosphate (AMPPCP), and cyclopiazonic acid (CPA). The structure determined at 2.5A resolution leads to a significantly revised model of CPA binding when compared with earlier reports. It shows that a divalent metal ion is required for CPA binding through coordination of the tetramic acid moiety at a characteristic kink of the M1 helix found in all P-type ATPase structures, which is expected to be part of the cytoplasmic cation access pathway. Our model is consistent with the biochemical data on CPA function and provides new measures in structure-based drug design targeting Ca(2+)-ATPases, e.g. from pathogens. We also present an extended structural basis of ATP modulation pinpointing key residues at or near the ATP binding site. A structural comparison to the Na(+),K(+)-ATPase reveals that the Phe(93) side chain occupies the equivalent binding pocket of the CPA site in SERCA, suggesting an important role of this residue in stabilization of the potassium-occluded E2 state of Na(+),K(+)-ATPase.

PubMed: 19289472
DOI: 10.1074/jbc.C900031200

実験手法

X-RAY DIFFRACTION (2.55 Å)