3FOE

Structural insight into the quinolone-DNA cleavage complex of type IIA topoisomerases

3FOE の概要

• エントリーDOI: 10.2210/pdb3foe/pdb
• 関連するPDBエントリー: 2NOV 3FOF
• 分子名称: DNA topoisomerase 4 subunit A, DNA topoisomerase 4 subunit B, DNA (5'-D(P*AP*CP*CP*AP*AP*GP*GP*TP*CP*AP*TP*GP*AP*AP*T)-3'), ... (7 entities in total)
• 機能のキーワード: quinolone, topoisomerase, dna, protein-dna cleavage complex, streptococcus pneumoniae, clinafloxacin, cell membrane, dna-binding, isomerase, membrane, atp-binding, nucleotide-binding, isomerase-dna complex, isomerase/dna
• 由来する生物種: Streptococcus pneumoniae; 詳細
• 細胞内の位置: Cell membrane; Peripheral membrane protein (By similarity): P72525
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 195299.44

構造登録者

Laponogov, I.,Sohi, M.K.,Veselkov, D.A.,Pan, X.-S.,Sawhney, R.,Thompson, A.W.,McAuley, K.E.,Fisher, L.M.,Sanderson, M.R. (登録日: 2008-12-30, 公開日: 2009-02-24, 最終更新日: 2023-11-01)

主引用文献

Laponogov, I.,Sohi, M.K.,Veselkov, D.A.,Pan, X.-S.,Sawhney, R.,Thompson, A.W.,McAuley, K.E.,Fisher, L.M.,Sanderson, M.R.
Structural insight into the quinolone-DNA cleavage complex of type IIA topoisomerases
Nat.Struct.Mol.Biol., 16:667-669, 2009

PubMed Abstract: Type II topoisomerases alter DNA topology by forming a covalent DNA-cleavage complex that allows DNA transport through a double-stranded DNA break. We present the structures of cleavage complexes formed by the Streptococcus pneumoniae ParC breakage-reunion and ParE TOPRIM domains of topoisomerase IV stabilized by moxifloxacin and clinafloxacin, two antipneumococcal fluoroquinolones. These structures reveal two drug molecules intercalated at the highly bent DNA gate and help explain antibacterial quinolone action and resistance.

PubMed: 19448616
DOI: 10.1038/nsmb.1604

実験手法

X-RAY DIFFRACTION (4.001 Å)