3FNS

Crystal structure of histo-aspartic protease (HAP) from Plasmodium Falciparum

3FNS の概要

• エントリーDOI: 10.2210/pdb3fns/pdb
• 関連するPDBエントリー: 3FNT 3FNU
• 分子名称: HAP protein, ZINC ION (3 entities in total)
• 機能のキーワード: histo-aspartic protease, hap, plasmepsin, aspartic protease, hormone, hydrolase
• 由来する生物種: Plasmodium falciparum 3D7
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 75262.95

構造登録者

Bhaumik, P.,Gustchina, A.,Wlodawer, A. (登録日: 2008-12-26, 公開日: 2009-05-12, 最終更新日: 2024-10-09)

主引用文献

Bhaumik, P.,Xiao, H.,Parr, C.L.,Kiso, Y.,Gustchina, A.,Yada, R.Y.,Wlodawer, A.
Crystal structures of the histo-aspartic protease (HAP) from Plasmodium falciparum.
J.Mol.Biol., 388:520-540, 2009

PubMed Abstract: The structures of recombinant histo-aspartic protease (HAP) from malaria-causing parasite Plasmodium falciparum as apoenzyme and in complex with two inhibitors, pepstatin A and KNI-10006, were solved at 2.5-, 3.3-, and 3.05-A resolutions, respectively. In the apoenzyme crystals, HAP forms a tight dimer not seen previously in any aspartic protease. The interactions between the monomers affect the conformation of two flexible loops, the functionally important "flap" (residues 70-83) and its structural equivalent in the C-terminal domain (residues 238-245), as well as the orientation of helix 225-235. The flap is found in an open conformation in the apoenzyme. Unexpectedly, the active site of the apoenzyme contains a zinc ion tightly bound to His32 and Asp215 from one monomer and to Glu278A from the other monomer, with the coordination of Zn resembling that seen in metalloproteases. The flap is closed in the structure of the pepstatin A complex, whereas it is open in the complex with KNI-10006. Although the binding mode of pepstatin A is significantly different from that in other pepsin-like aspartic proteases, its location in the active site makes unlikely the previously proposed hypothesis that HAP is a serine protease. The binding mode of KNI-10006 is unusual compared with the binding of other inhibitors from the KNI series to aspartic proteases. The novel features of the HAP active site could facilitate design of specific inhibitors used in the development of antimalarial drugs.

PubMed: 19285084
DOI: 10.1016/j.jmb.2009.03.011

実験手法

X-RAY DIFFRACTION (2.5 Å)