3FMG

Structure of rotavirus outer capsid protein VP7 trimer in complex with a neutralizing Fab

3FMG の概要

• エントリーDOI: 10.2210/pdb3fmg/pdb
• 分子名称: Fab of neutralizing antibody 4F8, light chain, Fab of neutralizing antibody 4F8, heavy chain, Glycoprotein VP7, ... (4 entities in total)
• 機能のキーワード: antibody-antigen complex, calcium dependent trimer, antiparallel beta-sandwich, jelly roll, alpha-beta-alpha sandwich, rossmann fold, capsid protein, endoplasmic reticulum, glycoprotein, membrane, transmembrane, virion, immune system
• 由来する生物種: Simian rotavirus; 詳細
• 細胞内の位置: Virion (By similarity): P12476
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 78281.81

構造登録者

Aoki, S.T.,Settembre, E.C.,Trask, S.D.,Greenberg, H.B.,Harrison, S.C.,Dormitzer, P.R. (登録日: 2008-12-22, 公開日: 2009-06-23, 最終更新日: 2024-11-27)

主引用文献

Aoki, S.T.,Settembre, E.C.,Trask, S.D.,Greenberg, H.B.,Harrison, S.C.,Dormitzer, P.R.
Structure of rotavirus outer-layer protein VP7 bound with a neutralizing Fab.
Science, 324:1444-1447, 2009

PubMed Abstract: Rotavirus outer-layer protein VP7 is a principal target of protective antibodies. Removal of free calcium ions (Ca2+) dissociates VP7 trimers into monomers, releasing VP7 from the virion, and initiates penetration-inducing conformational changes in the other outer-layer protein, VP4. We report the crystal structure at 3.4 angstrom resolution of VP7 bound with the Fab fragment of a neutralizing monoclonal antibody. The Fab binds across the outer surface of the intersubunit contact, which contains two Ca2+ sites. Mutations that escape neutralization by other antibodies suggest that the same region bears the epitopes of most neutralizing antibodies. The monovalent Fab is sufficient to neutralize infectivity. We propose that neutralizing antibodies against VP7 act by stabilizing the trimer, thereby inhibiting the uncoating trigger for VP4 rearrangement. A disulfide-linked trimer is a potential subunit immunogen.

PubMed: 19520960
DOI: 10.1126/science.1170481

実験手法

X-RAY DIFFRACTION (3.4 Å)