3FM3

Crystal structure of an Encephalitozoon cuniculi methionine aminopeptidase type 2

「2NW5」から置き換えられました

3FM3 の概要

• エントリーDOI: 10.2210/pdb3fm3/pdb
• 関連するPDBエントリー: 3FMQ 3FMR
• 分子名称: Methionine aminopeptidase 2, FE (III) ION, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: methionine aminopeptidase type2, metap2, encephalitozoon cuniculi, structural genomics, psi-2, protein structure initiative, new york sgx research center for structural genomics, nysgxrc, aminopeptidase, cobalt, hydrolase, metal-binding, protease
• 由来する生物種: Encephalitozoon cuniculi
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 80570.45

構造登録者

Alvarado, J.J.,Russell, M.,Zhang, A.,Adams, J.,Toro, R.,Burley, S.K.,Weiss, L.M.,Almo, S.C.,New York SGX Research Center for Structural Genomics (NYSGXRC) (登録日: 2008-12-19, 公開日: 2009-01-13, 最終更新日: 2023-09-06)

主引用文献

Alvarado, J.J.,Nemkal, A.,Sauder, J.M.,Russell, M.,Akiyoshi, D.E.,Shi, W.,Almo, S.C.,Weiss, L.M.
Structure of a microsporidian methionine aminopeptidase type 2 complexed with fumagillin and TNP-470.
Mol.Biochem.Parasitol., 168:158-167, 2009

PubMed Abstract: Microsporidia are protists that have been reported to cause infections in both vertebrates and invertebrates. They have emerged as human pathogens particularly in patients that are immunosuppressed and cases of gastrointestinal infection, encephalitis, keratitis, sinusitis, myositis and disseminated infection are well described in the literature. While benzimidazoles are active against many species of microsporidia, these drugs do not have significant activity against Enterocytozoon bieneusi. Fumagillin and its analogues have been demonstrated to have activity invitro and in animal models of microsporidiosis and human infections due to E. bieneusi. Fumagillin and its analogues inhibit methionine aminopeptidase type 2. Encephalitozoon cuniculi MetAP2 (EcMetAP2) was cloned and expressed as an active enzyme using a baculovirus system. The crystal structure of EcMetAP2 was determined with and without the bound inhibitors fumagillin and TNP-470. This structure classifies EcMetAP2 as a member of the MetAP2c family. The EcMetAP2 structure was used to generate a homology model of the E. bieneusi MetAP2. Comparison of microsporidian MetAP2 structures with human MetAP2 provides insights into the design of inhibitors that might exhibit specificity for microsporidian MetAP2.

PubMed: 19660503
DOI: 10.1016/j.molbiopara.2009.07.008

実験手法

X-RAY DIFFRACTION (2.18 Å)