3FJ5

Crystal structure of the c-src-SH3 domain

3FJ5 の概要

• エントリーDOI: 10.2210/pdb3fj5/pdb
• 分子名称: Proto-oncogene tyrosine-protein kinase Src, TRIETHYLENE GLYCOL, SULFATE ION, ... (7 entities in total)
• 機能のキーワード: beta shandwich, transferase, atp-binding, kinase, lipoprotein, myristate, nucleotide-binding, phosphoprotein, proto-oncogene, sh2 domain, sh3 domain, tyrosine-protein kinase
• 由来する生物種: Gallus gallus (Chicken)
• 細胞内の位置: Cell membrane (By similarity): P00523
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 13978.31

構造登録者

Camara-Artigas, A. (登録日: 2008-12-14, 公開日: 2009-03-03, 最終更新日: 2023-11-01)

主引用文献

Morel, B.,Ruiz-Sanz, J.,Luque, I.
Intertwined dimeric structure for the SH3 domain of the c-Src tyrosine kinase induced by polyethylene glycol binding
Febs Lett., 583:749-753, 2009

PubMed Abstract: Here we report the first crystal structure of the SH3 domain of the cellular Src tyrosine kinase (c-Src-SH3) domain on its own. In the crystal two molecules of c-Src-SH3 exchange their -RT loops generating an intertwined dimer, in which the two SH3 units, preserving the binding site configuration, are oriented to allow simultaneous binding of two ligand molecules. The dimerization of c-Src-SH3 is induced, both in the crystal and in solution, by the binding of a PEG molecule at the dimer interface, indicating that this type of conformations are energetically close to the native structure. These results have important implications respect to in vivo oligomerization and amyloid aggregation.

PubMed: 19185573
DOI: 10.1016/j.febslet.2009.01.036

実験手法

X-RAY DIFFRACTION (1.65 Å)