3FD2

Crystal structure of mMsoI/DNA complex with calcium

3FD2 の概要

• エントリーDOI: 10.2210/pdb3fd2/pdb
• 分子名称: Site-specific DNA endonuclease I-MsoI, 5'-D(*GP*CP*AP*GP*AP*AP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*CP*CP*G)-3', 5'-D(*CP*GP*GP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*TP*TP*CP*TP*GP*C)-3', ... (5 entities in total)
• 機能のキーワード: protein-dna complex, chloroplast, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Monomastix sp. (strain OKE-1); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 56846.66

構造登録者

Li, H.,Monnat, R.J. (登録日: 2008-11-24, 公開日: 2009-06-30, 最終更新日: 2023-09-06)

主引用文献

Li, H.,Pellenz, S.,Ulge, U.,Stoddard, B.L.,Monnat, R.J.
Generation of single-chain LAGLIDADG homing endonucleases from native homodimeric precursor proteins.
Nucleic Acids Res., 37:1650-1662, 2009

PubMed Abstract: Homing endonucleases (HEs) cut long DNA target sites with high specificity to initiate and target the lateral transfer of mobile introns or inteins. This high site specificity of HEs makes them attractive reagents for gene targeting to promote DNA modification or repair. We have generated several hundred catalytically active, monomerized versions of the well-characterized homodimeric I-CreI and I-MsoI LAGLIDADG family homing endonuclease (LHE) proteins. Representative monomerized I-CreI and I-MsoI proteins (collectively termed mCreIs or mMsoIs) were characterized in detail by using a combination of biochemical, biophysical and structural approaches. We also demonstrated that both mCreI and mMsoI proteins can promote cleavage-dependent recombination in human cells. The use of single chain LHEs should simplify gene modification and targeting by requiring the expression of a single small protein in cells, rather than the coordinate expression of two separate protein coding genes as is required when using engineered heterodimeric zinc finger or homing endonuclease proteins.

PubMed: 19153140
DOI: 10.1093/nar/gkp004

実験手法

X-RAY DIFFRACTION (2.69 Å)