3F5M

Crystal Structure of ATP-Bound Phosphofructokinase from Trypanosoma brucei

3F5M の概要

• エントリーDOI: 10.2210/pdb3f5m/pdb
• 関連するPDBエントリー: 1MTO 1PFK 2HIG 2PFK 3PFK 4PFK
• 分子名称: 6-phospho-1-fructokinase (ATP-dependent phosphofructokinase), ADENOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ... (7 entities in total)
• 機能のキーワード: 6-phospho-1-fructokinase, glycolysis, atp binding, kinase activity, atp-binding, kinase, nucleotide-binding, transferase
• 由来する生物種: Trypanosoma brucei
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 216847.24

構造登録者

McNae, I.W.,Martinez-Oyanedel, J.,Keillor, J.W.,Michels, P.A.M.,Fothergill-Gilmore, L.A.,Walkinshaw, M.D. (登録日: 2008-11-04, 公開日: 2008-11-25, 最終更新日: 2023-11-01)

主引用文献

McNae, I.W.,Martinez-Oyanedel, J.,Keillor, J.W.,Michels, P.A.,Fothergill-Gilmore, L.A.,Walkinshaw, M.D.
The crystal structure of ATP-bound phosphofructokinase from Trypanosoma brucei reveals conformational transitions different from those of other phosphofructokinases.
J.Mol.Biol., 385:1519-1533, 2009

PubMed Abstract: The crystal structure of the ATP-bound form of the tetrameric phosphofructokinase (PFK) from Trypanosoma brucei enables detailed comparisons to be made with the structures of the apoenzyme form of the same enzyme, as well as with those of bacterial ATP-dependent and PP(i)-dependent PFKs. The active site of T. brucei PFK (which is strictly ATP-dependent but belongs to the PP(i)-dependent family by sequence similarities) is a chimera of the two types of PFK. In particular, the active site of T. brucei PFK possesses amino acid residues and structural features characteristic of both types of PFK. Conformational changes upon ATP binding are observed that include the opening of the active site to accommodate the two substrates, MgATP and fructose 6-phosphate, and a dramatic ordering of the C-terminal helices, which act like reaching arms to hold the tetramer together. These conformational transitions are fundamentally different from those of other ATP-dependent PFKs. The substantial differences in structure and mechanism of T. brucei PFK compared with bacterial and mammalian PFKs give optimism for the discovery of species-specific drugs for the treatment of diseases caused by protist parasites of the trypanosomatid family.

PubMed: 19084537
DOI: 10.1016/j.jmb.2008.11.047

実験手法

X-RAY DIFFRACTION (2.7 Å)