3F5A

Crystal structure of Toxoplasma gondii micronemal protein 1 bound to 3'SiaLacNAc1-3

3F5A の概要

• エントリーDOI: 10.2210/pdb3f5a/pdb
• 関連するPDBエントリー: 2JH1 2JH7 2JHD 3F53 3F5E
• 分子名称: Micronemal protein 1, N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose, CHLORIDE ION, ... (6 entities in total)
• 機能のキーワード: cellular adhesion, micronemal protein, toxoplasma gondii, carbohydrate, fluorine, cell adhesion, cytoplasmic vesicle, lectin, virulence
• 由来する生物種: Toxoplasma gondii
• 細胞内の位置: Cytoplasmic vesicle, secretory vesicle, microneme: O00834
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28908.89

構造登録者

Garnett, J.A.,Liu, Y.,Feizi, T.,Matthews, S.J. (登録日: 2008-11-03, 公開日: 2009-07-28, 最終更新日: 2024-10-09)

主引用文献

Garnett, J.A.,Liu, Y.,Leon, E.,Allman, S.A.,Friedrich, N.,Saouros, S.,Curry, S.,Soldati-Favre, D.,Davis, B.G.,Feizi, T.,Matthews, S.
Detailed insights from microarray and crystallographic studies into carbohydrate recognition by microneme protein 1 (MIC1) of Toxoplasma gondii.
Protein Sci., 18:1935-1947, 2009

PubMed Abstract: The intracellular protozoan Toxoplasma gondii is among the most widespread parasites. The broad host cell range of the parasite can be explained by carbohydrate microarray screening analyses that have demonstrated the ability of the T. gondii adhesive protein, TgMIC1, to bind to a wide spectrum of sialyl oligosaccharide ligands. Here, we investigate by further microarray analyses in a dose-response format the differential binding of TgMIC1 to 2-3- and 2-6-linked sialyl carbohydrates. Interestingly, two novel synthetic fluorinated analogs of 3'SiaLacNAc(1-4) and 3'SiaLacNAc(1-3) were identified as highly potent ligands. To understand the structural basis of the carbohydrate binding specificity of TgMIC1, we have determined the crystal structures of TgMIC1 micronemal adhesive repeat (MAR)-region (TgMIC1-MARR) in complex with five sialyl-N-acetyllactosamine analogs. These crystal structures have revealed a specific, water-mediated hydrogen bond network that accounts for the preferential binding of TgMIC1-MARR to arrayed 2-3-linked sialyl oligosaccharides and the high potency of the fluorinated analogs. Furthermore, we provide strong evidence for the first observation of a C--F...H--O hydrogen bond within a lectin-carbohydrate complex. Finally, detailed comparison with other oligosaccharide-protein complexes in the Protein Data Bank (PDB) reveals a new family of sialic-acid binding sites from lectins in parasites, bacteria, and viruses.

PubMed: 19593815
DOI: 10.1002/pro.204

実験手法

X-RAY DIFFRACTION (2 Å)