3F2E

Crystal structure of Yellowstone SIRV coat protein C-terminus

Summary for 3F2E

• Entry DOI: 10.2210/pdb3f2e/pdb
• Descriptor: SIRV coat protein, CITRIC ACID (3 entities in total)
• Functional Keywords: four helix bundle, virus coat protein, viral protein
• Biological source: Sulfolobus islandicus rudivirus 1 variant YNP
• Total number of polymer chains: 1
• Total formula weight: 10690.97

Authors

Taurog, R.E.,Szymczyna, B.R.,Williamson, J.R.,Johnson, J.E. (deposition date: 2008-10-29, release date: 2009-04-21, Last modification date: 2024-04-03)

Primary citation

Szymczyna, B.R.,Taurog, R.E.,Young, M.J.,Snyder, J.C.,Johnson, J.E.,Williamson, J.R.
Synergy of NMR, computation, and X-ray crystallography for structural biology.
Structure, 17:499-507, 2009

PubMed Abstract: NMR spectroscopy and X-ray crystallography are currently the two most widely applied methods for the determination of macromolecular structures at high resolution. More recently, significant advances have been made in algorithms for the de novo prediction of protein structure, and, in favorable cases, the predicted models agree extremely well with experimentally determined structures. Here, we demonstrate a synergistic combination of NMR spectroscopy, de novo structure prediction, and X-ray crystallography in an effective overall strategy for rapidly determining the structure of the coat protein C-terminal domain from the Sulfolobus islandicus rod-shaped virus (SIRV). This approach takes advantage of the most accessible aspects of each structural technique and may be widely applicable for structure determination.

PubMed: 19368883
DOI: 10.1016/j.str.2009.03.001

Experimental method

X-RAY DIFFRACTION (1.668 Å)