3F2E

Crystal structure of Yellowstone SIRV coat protein C-terminus

3F2E の概要

• エントリーDOI: 10.2210/pdb3f2e/pdb
• 分子名称: SIRV coat protein, CITRIC ACID (3 entities in total)
• 機能のキーワード: four helix bundle, virus coat protein, viral protein
• 由来する生物種: Sulfolobus islandicus rudivirus 1 variant YNP
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10690.97

構造登録者

Taurog, R.E.,Szymczyna, B.R.,Williamson, J.R.,Johnson, J.E. (登録日: 2008-10-29, 公開日: 2009-04-21, 最終更新日: 2024-04-03)

主引用文献

Szymczyna, B.R.,Taurog, R.E.,Young, M.J.,Snyder, J.C.,Johnson, J.E.,Williamson, J.R.
Synergy of NMR, computation, and X-ray crystallography for structural biology.
Structure, 17:499-507, 2009

PubMed Abstract: NMR spectroscopy and X-ray crystallography are currently the two most widely applied methods for the determination of macromolecular structures at high resolution. More recently, significant advances have been made in algorithms for the de novo prediction of protein structure, and, in favorable cases, the predicted models agree extremely well with experimentally determined structures. Here, we demonstrate a synergistic combination of NMR spectroscopy, de novo structure prediction, and X-ray crystallography in an effective overall strategy for rapidly determining the structure of the coat protein C-terminal domain from the Sulfolobus islandicus rod-shaped virus (SIRV). This approach takes advantage of the most accessible aspects of each structural technique and may be widely applicable for structure determination.

PubMed: 19368883
DOI: 10.1016/j.str.2009.03.001

実験手法

X-RAY DIFFRACTION (1.668 Å)