3F1I

Human ESCRT-0 Core Complex

3F1I の概要

• エントリーDOI: 10.2210/pdb3f1i/pdb
• 分子名称: Hepatocyte growth factor-regulated tyrosine kinase substrate, Signal transducing adapter molecule 1 (3 entities in total)
• 機能のキーワード: hgs, stam, escrt, ubiquitin, mvb, endosome, membrane, metal-binding, phosphoprotein, protein transport, transport, zinc-finger, sh3 domain, protein binding
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm: O14964; Cytoplasm (Probable): Q92783
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 29324.25

構造登録者

Ren, X.,Kloer, D.P.,Kim, Y.,Ghirlando, R.,Saidi, L.,Hummer, G.,Hurley, J.H. (登録日: 2008-10-28, 公開日: 2009-03-24, 最終更新日: 2023-12-27)

主引用文献

Ren, X.,Kloer, D.P.,Kim, Y.C.,Ghirlando, R.,Saidi, L.F.,Hummer, G.,Hurley, J.H.
Hybrid Structural Model of the Complete Human ESCRT-0 Complex.
Structure, 17:406-416, 2009

PubMed Abstract: The human Hrs and STAM proteins comprise the ESCRT-0 complex, which sorts ubiquitinated cell surface receptors to lysosomes for degradation. Here we report a model for the complete ESCRT-0 complex based on the crystal structure of the Hrs-STAM core complex, previously solved domain structures, hydrodynamic measurements, and Monte Carlo simulations. ESCRT-0 expressed in insect cells has a hydrodynamic radius of RH = 7.9 nm and is a 1:1 heterodimer. The 2.3 Angstroms crystal structure of the ESCRT-0 core complex reveals two domain-swapped GAT domains and an antiparallel two-stranded coiled-coil, similar to yeast ESCRT-0. ESCRT-0 typifies a class of biomolecular assemblies that combine structured and unstructured elements, and have dynamic and open conformations to ensure versatility in target recognition. Coarse-grained Monte Carlo simulations constrained by experimental RH values for ESCRT-0 reveal a dynamic ensemble of conformations well suited for diverse functions.

PubMed: 19278655
DOI: 10.1016/j.str.2009.01.012

実験手法

X-RAY DIFFRACTION (2.3 Å)