3EZW

Crystal Structure of a Hyperactive Escherichia coli Glycerol Kinase Mutant Gly230 --> Asp Obtained Using Microfluidic Crystallization Devices

Replaces:  2P3R

Summary for 3EZW

• Entry DOI: 10.2210/pdb3ezw/pdb
• Descriptor: Glycerol kinase, GLYCEROL, CHLORIDE ION, ... (6 entities in total)
• Functional Keywords: glycerol kinase, glycerol metabolism, allosteric regulation, microfluidics, in situ data collection, atp-binding, kinase, metal-binding, nucleotide-binding, transferase
• Biological source: Escherichia coli
• Total number of polymer chains: 8
• Total formula weight: 475860.76

Authors

Anderson, M.J.,DeLaBarre, B.,Dunten, P.,Brunger, A.T.,Quake, S.R. (deposition date: 2008-10-23, release date: 2008-11-04, Last modification date: 2023-09-06)

• Primary citation: Anderson, M.J.,DeLabarre, B.,Raghunathan, A.,Palsson, B.O.,Brunger, A.T.,Quake, S.R.; Crystal structure of a hyperactive Escherichia coli glycerol kinase mutant Gly230 --> Asp obtained using microfluidic crystallization devices.; Biochemistry, 46:5722-5731, 2007; PubMed: 17441732; DOI: 10.1021/bi700096p

Experimental method

X-RAY DIFFRACTION (2 Å)