3EYY

Structural basis for the specialization of Nur, a nickel-specific Fur homologue, in metal sensing and DNA recognition

3EYY の概要

• エントリーDOI: 10.2210/pdb3eyy/pdb
• 分子名称: Putative iron uptake regulatory protein, NICKEL (II) ION, ZINC ION, ... (7 entities in total)
• 機能のキーワード: nur, nickel-uptake regulator, d-domain, dimerization domain, db-domain, dna-binding domain, transport
• 由来する生物種: Streptomyces coelicolor
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 33488.45

構造登録者

Cha, S.-S.,An, Y.J. (登録日: 2008-10-22, 公開日: 2009-06-09, 最終更新日: 2023-12-27)

主引用文献

An, Y.J.,Ahn, B.-E.,Han, A.-R.,Kim, H.-M.,Chung, K.M.,Shin, J.-H.,Cho, Y.-B.,Roe, J.-H.,Cha, S.-S.
Structural basis for the specialization of Nur, a nickel-specific Fur homolog, in metal sensing and DNA recognition
Nucleic Acids Res., 37:3442-3451, 2009

PubMed Abstract: Nur, a member of the Fur family, is a nickel-responsive transcription factor that controls nickel homeostasis and anti-oxidative response in Streptomyces coelicolor. Here we report the 2.4-A resolution crystal structure of Nur. It contains a unique nickel-specific metal site in addition to a nonspecific common metal site. The identification of the 6-5-6 motif of the Nur recognition box and a Nur/DNA complex model reveals that Nur mainly interacts with terminal bases of the palindrome on complex formation. This contrasts with more distributed contacts between Fur and the n-1-n type of the Fur-binding motif. The disparity between Nur and Fur in the conformation of the S1-S2 sheet in the DNA-binding domain can explain their different DNA-recognition patterns. Furthermore, the fact that the specificity of Nur in metal sensing and DNA recognition is conferred by the specific metal site suggests that its introduction drives the evolution of Nur orthologs in the Fur family.

PubMed: 19336416
DOI: 10.1093/nar/gkp198

実験手法

X-RAY DIFFRACTION (2.4 Å)