3EYC

New crystal structure of human tear lipocalin in complex with 1,4-butanediol in space group P21

3EYC の概要

• エントリーDOI: 10.2210/pdb3eyc/pdb
• 関連するPDBエントリー: 1xki
• 分子名称: Lipocalin-1, 1,4-BUTANEDIOL (3 entities in total)
• 機能のキーワード: transport protein, ligand-binding protein, beta-barrel, secreted, sensory transduction, taste, transport
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P31025
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 72157.20

構造登録者

Breustedt, D.A.,Keil, L.,Skerra, A. (登録日: 2008-10-20, 公開日: 2009-10-06, 最終更新日: 2024-10-30)

主引用文献

Breustedt, D.A.,Chatwell, L.,Skerra, A.
A new crystal form of human tear lipocalin reveals high flexibility in the loop region and induced fit in the ligand cavity
Acta Crystallogr.,Sect.D, 65:1118-1125, 2009

PubMed Abstract: Tear lipocalin (TLC) with the bound artificial ligand 1,4-butanediol has been crystallized in space group P2(1) with four protein molecules in the asymmetric unit and its X-ray structure has been solved at 2.6 A resolution. TLC is a member of the lipocalin family that binds ligands with diverse chemical structures, such as fatty acids, phospholipids and cholesterol as well as microbial siderophores and the antibiotic rifampin. Previous X-ray structural analysis of apo TLC crystallized in space group C2 revealed a rather large bifurcated ligand pocket and a partially disordered loop region at the entrace to the cavity. Analysis of the P2(1) crystal form uncovered major conformational changes (i) in beta-strands B, C and D, (ii) in loops 1, 2 and 4 at the open end of the beta-barrel and (iii) in the extended C-terminal segment, which is attached to the beta-barrel via a disulfide bridge. The structural comparison indicates high conformational plasticity of the loop region as well as of deeper parts of the ligand pocket, thus allowing adaptation to ligands that differ vastly in size and shape. This illustrates a mechanism for promiscuity in ligand recognition which may also be relevant for some other physiologically important members of the lipocalin protein family.

PubMed: 19770509
DOI: 10.1107/S0907444909031011

実験手法

X-RAY DIFFRACTION (2.6 Å)