Loading
PDBj
メニューPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

3EVR

Crystal structure of Calcium bound monomeric GCAMP2

3EVR の概要
エントリーDOI10.2210/pdb3evr/pdb
関連するPDBエントリー3EVP 3EVU 3EWE
分子名称Myosin light chain kinase, Green fluorescent protein, Calmodulin-1 chimera, CALCIUM ION (3 entities in total)
機能のキーワードgcamp2, calcium sensor, gfp, calmodulin, m13, signaling protein
由来する生物種Aequorea victoria (Jellyfish)
詳細
細胞内の位置Cytoplasm, cytoskeleton, spindle: P0DP29
タンパク質・核酸の鎖数1
化学式量合計46568.14
構造登録者
Wang, Q.,Shui, B.,Kotlikoff, M.I.,Sondermann, H. (登録日: 2008-10-13, 公開日: 2008-12-09, 最終更新日: 2024-11-06)
主引用文献Wang, Q.,Shui, B.,Kotlikoff, M.I.,Sondermann, H.
Structural Basis for Calcium Sensing by GCaMP2.
Structure, 16:1817-1827, 2008
Cited by
PubMed Abstract: Genetically encoded Ca(2+) indicators are important tools that enable the measurement of Ca(2+) dynamics in a physiologically relevant context. GCaMP2, one of the most robust indicators, is a circularly permutated EGFP (cpEGFP)/M13/calmodulin (CaM) fusion protein that has been successfully used for studying Ca(2+) fluxes in vivo in the heart and vasculature of transgenic mice. Here we describe crystal structures of bright and dim states of GCaMP2 that reveal a sophisticated molecular mechanism for Ca(2+) sensing. In the bright state, CaM stabilizes the fluorophore in an ionized state similar to that observed in EGFP. Mutational analysis confirmed critical interactions between the fluorophore and elements of the fused peptides. Solution scattering studies indicate that the Ca(2+)-free form of GCaMP2 is a compact, predocked state, suggesting a molecular basis for the relatively rapid signaling kinetics reported for this indicator. These studies provide a structural basis for the rational design of improved Ca(2+)-sensitive probes.
PubMed: 19081058
DOI: 10.1016/j.str.2008.10.008
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2 Å)
構造検証レポート
Validation report summary of 3evr
検証レポート(詳細版)ダウンロードをダウンロード

227111

件を2024-11-06に公開中

PDB statisticsPDBj update infoContact PDBjnumon