3EU1

Crystal Structure determination of goat hemoglobin (Capra hircus) at 3 angstrom resolution

3EU1 の概要

• エントリーDOI: 10.2210/pdb3eu1/pdb
• 関連するPDBエントリー: 2qu0 2ri4 3cy5 3d1a 3d4x
• 分子名称: Hemoglobin subunit alpha-1/2, Hemoglobin subunit beta-A, PROTOPORPHYRIN IX CONTAINING FE (3 entities in total)
• 機能のキーワード: low oxygen affinity, capra hircus, hemoglobin, allosteric mechanism, 2, 3-diphospho glycerate, oxygen storage, oxygen transport
• 由来する生物種: Capra hircus (domestic goat, goats); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 64671.07

構造登録者

Sathya Moorthy, P.,Neelagandan, K.,Balasubramanian, M.,Ponnuswamy, M.N. (登録日: 2008-10-09, 公開日: 2009-11-10, 最終更新日: 2023-11-01)

主引用文献

Sathya Moorthy, P.,Neelagandan, K.,Balasubramanian, M.,Ponnuswamy, M.N.
Purification, crystallization and preliminary X-ray diffraction studies on goat (Capra hircus) hemoglobin - a low oxygen affinity species
Protein Pept.Lett., 16:454-456, 2009

PubMed Abstract: Hemoglobin is a vital protein present in almost all higher species. It is a transport protein involved in carrying oxygen from lungs to tissues and carbon dioxide back to lungs by an intrinsically coordinated manner. Even though a good amount of work has been carried out in this direction there exists scarcity of structural insight on low oxygen affinity species. Attempts are being made to unravel the structural insight of this low oxygen affinity species. Goat blood plasma was collected, treated with EDTA to avoid blood clotting and purification was accomplished using DEAE-anion chromatographic column. The goat hemoglobin was crystallized using 50mM of phosphate buffer at pH 6.7 with 1M NaCl and PEG 3350 as precipitant by hanging drop vapor diffusion method. Crystals obtained are screened and suitable crystals are taken for data collection using mar345dtb as image plate detector system. Goat hemoglobin crystal diffracted up to 2.61 A resolution. Goat hemoglobin crystallizes in orthorhombic space group P212(1)2(1) as a whole biological molecule in the asymmetric unit with cell dimensions a=53.568A, b=67.365A, c=154.183A.

PubMed: 19356147
DOI: 10.2174/092986609787847992

実験手法

X-RAY DIFFRACTION (3 Å)