3EOX

High quality structure of cleaved PAI-1-stab

Summary for 3EOX

• Entry DOI: 10.2210/pdb3eox/pdb
• Related: 1DB2 9PAI
• Descriptor: Plasminogen activator inhibitor 1 (2 entities in total)
• Functional Keywords: cleaved plasminogen activator inhibitor-1, pai-1, serpin, stabilized, glycoprotein, plasminogen activation, protease inhibitor, secreted, serine protease inhibitor, blood clotting
• Biological source: Homo sapiens (Human)
• Cellular location: Secreted: P05121
• Total number of polymer chains: 1
• Total formula weight: 42751.01

Authors

Dewilde, M.,Declerck, P.J.,Rabijns, A.,Strelkov, S.V. (deposition date: 2008-09-29, release date: 2009-02-17, Last modification date: 2023-11-01)

Primary citation

Dewilde, M.,Strelkov, S.V.,Rabijns, A.,Declerck, P.J.
High quality structure of cleaved PAI-1-stab.
J.Struct.Biol., 165:126-132, 2009

PubMed Abstract: Here we report the crystal structure of a stablilized plasminogen activator inhibitor-1 variant (PAI-1-N150H-K154T-Q301P-Q319L-M354I (PAI-1-stab)) that shows a cleavage within the reactive centre loop. The new structure is of superior quality compared to the previously determined structure of the cleaved PAI-1-A335P mutant. We present a detailed comparison of the two structures and also compare them with the structure of the active PAI-1-stab. The structural data give important insights into the working mechanism of PAI-1 and also explain the role of various stabilizing mutations.

PubMed: 19059484
DOI: 10.1016/j.jsb.2008.11.001

Experimental method

X-RAY DIFFRACTION (2.61 Å)