3ENB

Crystal Structure of PRP8 core domain IV

3ENB の概要

• エントリーDOI: 10.2210/pdb3enb/pdb
• 分子名称: Pre-mRNA-processing-splicing factor 8 (2 entities in total)
• 機能のキーワード: prp8 domain iv, beta finger, rnase h, spliceosome, u5-220k, disease mutation, mrna processing, mrna splicing, nucleus, phosphoprotein, retinitis pigmentosa, rna-binding, sensory transduction, vision, rna binding protein
• 由来する生物種: Homo sapiens
• 細胞内の位置: Nucleus speckle : Q6P2Q9
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 51077.57

構造登録者

Schellenberg, M.J.,Ritchie, D.B.,MacMillan, A.M. (登録日: 2008-09-25, 公開日: 2008-10-07, 最終更新日: 2024-02-21)

主引用文献

Ritchie, D.B.,Schellenberg, M.J.,Gesner, E.M.,Raithatha, S.A.,Stuart, D.T.,Macmillan, A.M.
Structural elucidation of a PRP8 core domain from the heart of the spliceosome.
Nat.Struct.Mol.Biol., 15:1199-1205, 2008

PubMed Abstract: The spliceosome is a complex ribonucleoprotein (RNP) particle containing five RNAs and more than 100 associated proteins. One of these proteins, PRP8, has been shown to interact directly with the splice sites and branch region of precursor-mRNAs (pre-mRNAs) and spliceosomal RNAs associated with catalysis of the two steps of splicing. The 1.85-A X-ray structure of the core of PRP8 domain IV, implicated in key spliceosomal interactions, reveals a bipartite structure that includes the presence of an RNase H fold linked to a five-helix assembly. Analysis of mutant yeast alleles and cross-linking results in the context of this structure, coupled with RNA binding studies, suggests that domain IV forms a surface that interacts directly with the RNA structures at the catalytic core of the spliceosome.

PubMed: 18836455
DOI: 10.1038/nsmb.1505

実験手法

X-RAY DIFFRACTION (1.85 Å)