3EKI

Structural insights of the Mycoplasma hyorhinis protein Mh-p37: A putative thiamine pyrophosphate transporter

3EKI の概要

• エントリーDOI: 10.2210/pdb3eki/pdb
• 関連するPDBエントリー: 3E78 3E79
• 分子名称: High affinity transport system protein p37, THIAMINE DIPHOSPHATE, GLYCEROL, ... (6 entities in total)
• 機能のキーワード: mycoplasma, p37, tpp, cell membrane, lipoprotein, membrane, transport, transport protein, palmitate, extracytoplasmic thiamine binding lipoprotein, cypl, tpp binding protein
• 由来する生物種: Mycoplasma hyorhinis
• 細胞内の位置: Cell membrane; Lipid-anchor: P15363
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 47274.71

構造登録者

Sippel, K.H.,Robbins, A.H.,Reutzel, R.,McKenna, R. (登録日: 2008-09-19, 公開日: 2009-06-23, 最終更新日: 2023-08-30)

主引用文献

Sippel, K.H.,Robbins, A.H.,Reutzel, R.,Boehlein, S.K.,Namiki, K.,Goodison, S.,Agbandje-McKenna, M.,Rosser, C.J.,McKenna, R.
Structural insights into the extracytoplasmic thiamine-binding lipoprotein p37 of Mycoplasma hyorhinis
J.Bacteriol., 191:2585-2592, 2009

PubMed Abstract: The Mycoplasma hyorhinis protein p37 has been implicated in tumorigenic transformation for more than 20 years. Though there are many speculations as to its function, based solely on sequence homology, the issue has remained unresolved. Presented here is the 1.6-A-resolution refined crystal structure of M. hyorhinis p37, renamed the extracytoplasmic thiamine-binding lipoprotein (Cypl). The structure shows thiamine pyrophosphate (TPP) and two calcium ions are bound to Cypl and give the first insights into possible functions of the Cypl-like family of proteins. Sequence alignments of Cypl-like proteins between several different species of mycoplasma show that the thiamine-binding site is likely conserved and structural alignments reveal the similarity of Cypl to various binding proteins. While the experimentally determined function of Cypl remains unknown, the structure shows that the protein is a TPP-binding protein, opening up many avenues for future mechanistic studies and making Cypl a possible target for combating mycoplasma infections and tumorigenic transformation.

PubMed: 19233924
DOI: 10.1128/JB.01680-08

実験手法

X-RAY DIFFRACTION (1.6 Å)