3EJG

Crystal structure of HCoV-229E X-domain

Summary for 3EJG

• Entry DOI: 10.2210/pdb3ejg/pdb
• Related: 3EJF 3EJG
• Descriptor: Non-structural protein 3 (2 entities in total)
• Functional Keywords: hcov 229e, human coronavirus, x-domain, macro domain, nsp3, adrp, hydrolase, ribosomal frameshifting, rna-binding
• Biological source: Human coronavirus 229E (HCoV-229E)
• Cellular location: Non-structural protein 3: Host membrane ; Multi-pass membrane protein . Non-structural protein 4: Host membrane ; Multi-pass membrane protein . Non-structural protein 6: Host membrane ; Multi-pass membrane protein . Non-structural protein 7: Host cytoplasm, host perinuclear region . Non-structural protein 8: Host cytoplasm, host perinuclear region . Non-structural protein 9: Host cytoplasm, host perinuclear region . Non-structural protein 10: Host cytoplasm, host perinuclear region . Helicase: Host endoplasmic reticulum-Golgi intermediate compartment . Uridylate-specific endoribonuclease: Host cytoplasm, host perinuclear region : P0C6X1
• Total number of polymer chains: 1
• Total formula weight: 21343.43

Authors

Piotrowski, Y.,Hansen, G.,Hilgenfeld, R. (deposition date: 2008-09-18, release date: 2008-09-30, Last modification date: 2024-02-21)

Primary citation

Piotrowski, Y.,Hansen, G.,Boomaars-van der Zanden, A.L.,Snijder, E.J.,Gorbalenya, A.E.,Hilgenfeld, R.
Crystal structures of the X-domains of a Group-1 and a Group-3 coronavirus reveal that ADP-ribose-binding may not be a conserved property.
Protein Sci., 18:6-16, 2009

PubMed Abstract: The polyproteins of coronaviruses are cleaved by viral proteases into at least 15 nonstructural proteins (Nsps). Consisting of five domains, Nsp3 is the largest of these (180-210 kDa). Among these domains, the so-called X-domain is believed to act as ADP-ribose-1''-phosphate phosphatase or to bind poly(ADP-ribose). However, here we show that the X-domain of Infectious Bronchitis Virus (strain Beaudette), a Group-3 coronavirus, fails to bind ADP-ribose. This is explained on the basis of the crystal structure of the protein, determined at two different pH values. For comparison, we also describe the crystal structure of the homologous X-domain from Human Coronavirus 229E, a Group-1 coronavirus, which does bind ADP-ribose.

PubMed: 19177346
DOI: 10.1002/pro.15

Experimental method

X-RAY DIFFRACTION (1.78 Å)