3EIP

CRYSTAL STRUCTURE OF COLICIN E3 IMMUNITY PROTEIN: AN INHIBITOR TO A RIBOSOME-INACTIVATING RNASE

Summary for 3EIP

• Entry DOI: 10.2210/pdb3eip/pdb
• Descriptor: PROTEIN (COLICIN E3 IMMUNITY PROTEIN), ZINC ION (3 entities in total)
• Functional Keywords: ribonuclease inhibitor, colicin, immune system
• Biological source: Escherichia coli str. K12 substr.
• Total number of polymer chains: 2
• Total formula weight: 19624.54

Authors

Li, C.,Zhao, D.,Djebli, A.,Shoham, M. (deposition date: 1999-03-29, release date: 1999-11-10, Last modification date: 2023-12-27)

Primary citation

Li, C.,Zhao, D.,Djebli, A.,Shoham, M.
Crystal structure of colicin E3 immunity protein: an inhibitor of a ribosome-inactivating RNase.
Structure Fold.Des., 7:1365-1372, 1999

PubMed Abstract: Colicins are antibiotic-like proteins of Escherichia coli that kill related strains. Colicin E3 acts as an RNase that specifically cleaves 16S rRNA, thereby inactivating the ribosomes in the infected cell. The producing organism is protected against colicin E3 by a specific inhibitor, the immunity protein Im3, which forms a tight 1:1 complex with colicin E3 and renders it inactive. Crystallographic studies on colicin E3 and Im3 have been undertaken to unravel the structural basis for the ribonucleolytic activity and its inhibition.

PubMed: 10574790
DOI: 10.1016/S0969-2126(00)80026-X

Experimental method

X-RAY DIFFRACTION (1.8 Å)