3EIP

CRYSTAL STRUCTURE OF COLICIN E3 IMMUNITY PROTEIN: AN INHIBITOR TO A RIBOSOME-INACTIVATING RNASE

3EIP の概要

• エントリーDOI: 10.2210/pdb3eip/pdb
• 分子名称: PROTEIN (COLICIN E3 IMMUNITY PROTEIN), ZINC ION (3 entities in total)
• 機能のキーワード: ribonuclease inhibitor, colicin, immune system
• 由来する生物種: Escherichia coli str. K12 substr.
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 19624.54

構造登録者

Li, C.,Zhao, D.,Djebli, A.,Shoham, M. (登録日: 1999-03-29, 公開日: 1999-11-10, 最終更新日: 2023-12-27)

主引用文献

Li, C.,Zhao, D.,Djebli, A.,Shoham, M.
Crystal structure of colicin E3 immunity protein: an inhibitor of a ribosome-inactivating RNase.
Structure Fold.Des., 7:1365-1372, 1999

PubMed Abstract: Colicins are antibiotic-like proteins of Escherichia coli that kill related strains. Colicin E3 acts as an RNase that specifically cleaves 16S rRNA, thereby inactivating the ribosomes in the infected cell. The producing organism is protected against colicin E3 by a specific inhibitor, the immunity protein Im3, which forms a tight 1:1 complex with colicin E3 and renders it inactive. Crystallographic studies on colicin E3 and Im3 have been undertaken to unravel the structural basis for the ribonucleolytic activity and its inhibition.

PubMed: 10574790
DOI: 10.1016/S0969-2126(00)80026-X

実験手法

X-RAY DIFFRACTION (1.8 Å)