3EEB

Structure of the V. cholerae RTX cysteine protease domain

3EEB の概要

• エントリーDOI: 10.2210/pdb3eeb/pdb
• 分子名称: RTX toxin RtxA, INOSITOL HEXAKISPHOSPHATE, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: v. cholerae, repeats-in-toxin, rtx, martx, cysteine protease, inositol hexakisphosphate, toxin
• 由来する生物種: Vibrio cholerae
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 46785.89

構造登録者

Lupardus, P.J.,Shen, A.,Bogyo, M.,Garcia, K.C. (登録日: 2008-09-04, 公開日: 2008-10-21, 最終更新日: 2024-02-21)

主引用文献

Lupardus, P.J.,Shen, A.,Bogyo, M.,Garcia, K.C.
Small molecule-induced allosteric activation of the Vibrio cholerae RTX cysteine protease domain
Science, 322:265-268, 2008

PubMed Abstract: Vibrio cholerae RTX (repeats in toxin) is an actin-disrupting toxin that is autoprocessed by an internal cysteine protease domain (CPD). The RTX CPD is efficiently activated by the eukaryote-specific small molecule inositol hexakisphosphate (InsP6), and we present the 2.1 angstrom structure of the RTX CPD in complex with InsP6. InsP6 binds to a conserved basic cleft that is distant from the protease active site. Biochemical and kinetic analyses of CPD mutants indicate that InsP6 binding induces an allosteric switch that leads to the autoprocessing and intracellular release of toxin-effector domains.

PubMed: 18845756
DOI: 10.1126/science.1162403

実験手法

X-RAY DIFFRACTION (2.1 Å)