3EBJ

Crystal structure of an avian influenza virus protein

3EBJ の概要

• エントリーDOI: 10.2210/pdb3ebj/pdb
• 分子名称: Polymerase acidic protein, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: avian influenza, polymerase, pa_n, phosphoprotein, transferase
• 由来する生物種: Influenza A virus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 120777.78

構造登録者

Yuan, P.,Bartlam, M.,Lou, Z.,Chen, S.,Rao, Z.,Liu, Y. (登録日: 2008-08-27, 公開日: 2009-02-10, 最終更新日: 2024-05-29)

主引用文献

Yuan, P.,Bartlam, M.,Lou, Z.,Chen, S.,Zhou, J.,He, X.,Lv, Z.,Ge, R.,Li, X.,Deng, T.,Fodor, E.,Rao, Z.,Liu, Y.
Crystal structure of an avian influenza polymerase PA(N) reveals an endonuclease active site
Nature, 458:909-913, 2009

PubMed Abstract: The heterotrimeric influenza virus polymerase, containing the PA, PB1 and PB2 proteins, catalyses viral RNA replication and transcription in the nucleus of infected cells. PB1 holds the polymerase active site and reportedly harbours endonuclease activity, whereas PB2 is responsible for cap binding. The PA amino terminus is understood to be the major functional part of the PA protein and has been implicated in several roles, including endonuclease and protease activities as well as viral RNA/complementary RNA promoter binding. Here we report the 2.2 ångström (A) crystal structure of the N-terminal 197 residues of PA, termed PA(N), from an avian influenza H5N1 virus. The PA(N) structure has an alpha/beta architecture and reveals a bound magnesium ion coordinated by a motif similar to the (P)DX(N)(D/E)XK motif characteristic of many endonucleases. Structural comparisons and mutagenesis analysis of the motif identified in PA(N) provide further evidence that PA(N) holds an endonuclease active site. Furthermore, functional analysis with in vivo ribonucleoprotein reconstitution and direct in vitro endonuclease assays strongly suggest that PA(N) holds the endonuclease active site and has critical roles in endonuclease activity of the influenza virus polymerase, rather than PB1. The high conservation of this endonuclease active site among influenza strains indicates that PA(N) is an important target for the design of new anti-influenza therapeutics.

PubMed: 19194458
DOI: 10.1038/nature07720

実験手法

X-RAY DIFFRACTION (2.2 Å)