3EB8

VirA

3EB8 の概要

• エントリーDOI: 10.2210/pdb3eb8/pdb
• 分子名称: Cysteine protease-like virA (2 entities in total)
• 機能のキーワード: beta sheet, alpha helix, hydrolase, protease, secreted, thiol protease, virulence
• 由来する生物種: Shigella flexneri
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 79745.83

構造登録者

Germane, K.L.,Spiller, B.W. (登録日: 2008-08-27, 公開日: 2008-09-30, 最終更新日: 2024-02-21)

主引用文献

Germane, K.L.,Ohi, R.,Goldberg, M.B.,Spiller, B.W.
Structural and functional studies indicate that Shigella VirA is not a protease and does not directly destabilize microtubules.
Biochemistry, 47:10241-10243, 2008

PubMed Abstract: VirA, an essential virulence factor in Shigella disease pathogenesis, is involved in the uptake, motility, and cell-to-cell spread of Shigella organisms within the human host. These functions have been attributed to a VirA protease activity and a mechanism of microtubule destruction via tubulin degradation [Yoshida, S., et al. (2006) Science 314, 985-989]. We report functional and crystallographic data indicating a novel VirA structure that lacks these activities but highlights the homology to the EspG virulence factor of pathogenic Escherichia coli.

PubMed: 18763811
DOI: 10.1021/bi801533k

実験手法

X-RAY DIFFRACTION (2.4 Å)