3E6E

Crystal structure of Alanine racemase from E.faecalis complex with cycloserine

3E6E の概要

• エントリーDOI: 10.2210/pdb3e6e/pdb
• 関連するPDBエントリー: 3E5P
• 分子名称: Alanine racemase, D-[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL]-N,O-CYCLOSERYLAMIDE (3 entities in total)
• 機能のキーワード: alr, scp, isomerase, pyridoxal phosphate
• 由来する生物種: Enterococcus faecalis (Streptococcus faecalis)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 124104.15

構造登録者

Hwang, K.Y.,Priyadarshi, A.,Lee, E.H.,Sung, M.W. (登録日: 2008-08-15, 公開日: 2009-08-18, 最終更新日: 2023-11-01)

主引用文献

Priyadarshi, A.,Lee, E.H.,Sung, M.W.,Nam, K.H.,Lee, W.H.,Kim, E.E.,Hwang, K.Y.
Structural insights into the alanine racemase from Enterococcus faecalis.
Biochim.Biophys.Acta, 1794:1030-1040, 2009

PubMed Abstract: Alanine racemase (AlaR) is a bacterial enzyme that belongs to the fold-type III group of pyridoxal 5'-phosphate (PLP)-dependent enzymes. AlaR catalyzes the interconversion between L- and D-alanine, which is important for peptidoglycan biosynthesis. This enzyme is common in prokaryotes, but absent in eukaryotes, which makes it an attractive target for the design of new antibacterial drugs. Here, we report the crystal structures of both the apoenzyme and the d-cycloserine (DCS) complex of AlaR from the pathogenic bacterium Enterococcus faecalis v583, at a resolution of 2.5 A. DCS is a suicide inhibitor of AlaR and, as such, serves as an antimicrobial agent and has been used to treat tuberculosis and urinary tract infection-related diseases, and makes several hydrogen bonds with the conserved active site residues, Tyr44 and Ser207, respectively. The apoenzyme crystal structure of AlaR consists of three monomers in the asymmetric unit, including a polyethylene glycol molecule in the dimer interface that surrounds one of the His 293 residues and also sits close to one side of the His 293 residue in the opposite monomer. Our results provide structural insights into AlaR that may be used for the development of new antibiotics targeting the alanine racemase in pathogenic bacteria.

PubMed: 19328247
DOI: 10.1016/j.bbapap.2009.03.006

実験手法

X-RAY DIFFRACTION (2.5 Å)