3E59

Crystal structure of the PvcA (PA2254) protein from Pseudomonas aeruginosa

3E59 の概要

• エントリーDOI: 10.2210/pdb3e59/pdb
• 分子名称: Pyoverdine biosynthesis protein PvcA, PHOSPHATE ION, TRIETHYLENE GLYCOL, ... (4 entities in total)
• 機能のキーワード: pvca, isonitrile, paerucumarin, 2-isocyano-6, 7-dihydroxycoumarin, transferase
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 150384.76

構造登録者

Gulick, A.M.,Drake, E.J. (登録日: 2008-08-13, 公開日: 2008-10-14, 最終更新日: 2024-02-21)

主引用文献

Drake, E.J.,Gulick, A.M.
Three-dimensional structures of Pseudomonas aeruginosa PvcA and PvcB, two proteins involved in the synthesis of 2-isocyano-6,7-dihydroxycoumarin.
J.Mol.Biol., 384:193-205, 2008

PubMed Abstract: The pvcABCD operon of Pseudomonas aeruginosa encodes four proteins (PA2254, PA2255, PA2256, and PA2257) that form a cluster that is responsible for the synthesis of a cyclized isocyano derivative of tyrosine. These proteins, which were identified originally as being responsible for a step in the maturation of the chromophore of the peptide siderophore pyoverdine, have been identified recently as belonging to a family of proteins that produce small organic isonitriles. We report that strains harboring a disruption in the pvcA or pvcB genes are able to grow in iron-depleted conditions and to produce pyoverdine. Additionally, we have determined the three-dimensional crystal structures of PvcA and PvcB. The structure of PvcA demonstrates a novel enzyme architecture that is built upon a Rossmann fold. We have analyzed the sequence conservation of enzymes within this family and identified six conserved motifs. These regions of the protein cluster around a putative active site cavity. The structure of the PvcB protein confirms it is a member of the Fe2+/alpha-ketoglutarate-dependent oxygenase family of enzymes. The active site of PvcB is compared to the structures of other family members and suggests that a conformational change to order several loops will accompany the binding of ligands.

PubMed: 18824174
DOI: 10.1016/j.jmb.2008.09.027

実験手法

X-RAY DIFFRACTION (2.1 Å)