3E50

Crystal structure of human insulin degrading enzyme in complex with transforming growth factor-alpha

3E50 の概要

• エントリーDOI: 10.2210/pdb3e50/pdb
• 関連するPDBエントリー: 3E4Z
• 分子名称: Insulin-degrading enzyme, Protransforming growth factor alpha, ZINC ION, ... (4 entities in total)
• 機能のキーワード: ide, tgf-alpha, cytoplasm, hydrolase, metal-binding, metalloprotease, polymorphism, protease, zinc, cell membrane, egf-like domain, glycoprotein, growth factor, lipoprotein, membrane, mitogen, palmitate, secreted, transmembrane, hydrolase-hormone complex, hydrolase/hormone
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Cytoplasm: P14735; Transforming growth factor alpha: Secreted, extracellular space. Protransforming growth factor alpha: Cell membrane; Single-pass type I membrane protein: P01135
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 240681.59

構造登録者

Guo, Q.,Manolopoulou, M.,Tang, W.-J. (登録日: 2008-08-12, 公開日: 2009-08-18, 最終更新日: 2024-02-21)

主引用文献

Guo, Q.,Manolopoulou, M.,Bian, Y.,Schilling, A.B.,Tang, W.J.
Molecular Basis for the Recognition and Cleavages of IGF-II, TGF-alpha, and Amylin by Human Insulin-Degrading Enzyme.
J.Mol.Biol., 395:430-443, 2010

PubMed Abstract: Insulin-degrading enzyme (IDE) is involved in the clearance of many bioactive peptide substrates, including insulin and amyloid-beta, peptides vital to the development of diabetes and Alzheimer's disease, respectively. IDE can also rapidly degrade hormones that are held together by intramolecular disulfide bond(s) without their reduction. Furthermore, IDE exhibits a remarkable ability to preferentially degrade structurally similar peptides such as the selective degradation of insulin-like growth factor (IGF)-II and transforming growth factor-alpha (TGF-alpha) over IGF-I and epidermal growth factor, respectively. Here, we used high-accuracy mass spectrometry to identify the cleavage sites of human IGF-II, TGF-alpha, amylin, reduced amylin, and amyloid-beta by human IDE. We also determined the structures of human IDE-IGF-II and IDE-TGF-alpha at 2.3 A and IDE-amylin at 2.9 A. We found that IDE cleaves its substrates at multiple sites in a biased stochastic manner. Furthermore, the presence of a disulfide bond in amylin allows IDE to cut at an additional site in the middle of the peptide (amino acids 18-19). Our amylin-bound IDE structure offers insight into how the structural constraint from a disulfide bond in amylin can alter IDE cleavage sites. Together with NMR structures of amylin and the IGF and epidermal growth factor families, our work also reveals the structural basis of how the high dipole moment of substrates complements the charge distribution of the IDE catalytic chamber for the substrate selectivity. In addition, we show how the ability of substrates to properly anchor their N-terminus to the exosite of IDE and undergo a conformational switch upon binding to the catalytic chamber of IDE can also contribute to the selective degradation of structurally related growth factors.

PubMed: 19896952
DOI: 10.1016/j.jmb.2009.10.072

実験手法

X-RAY DIFFRACTION (2.3 Å)