3E43

Q138F HincII bound to GTTAAC and cocrystallized with 2.5 mM MgCl2

3E43 の概要

• エントリーDOI: 10.2210/pdb3e43/pdb
• 関連するPDBエントリー: 1TX3 3E3Y 3E40 3E41 3E42 3E44 3E45
• 分子名称: Type-2 restriction enzyme HindII, 5'-D(*DGP*DCP*DCP*DGP*DGP*DTP*DTP*DAP*DAP*DCP*DCP*DGP*DGP*DC)-3', UNKNOWN ATOM OR ION, ... (4 entities in total)
• 機能のキーワード: protein-dna complex, endonuclease, indirect readout, hydrolase, nuclease, restriction system, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Haemophilus influenzae
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 68241.78

構造登録者

Horton, N.C.,Babic, A.C.,Little, E.J.,Manohar, V.M. (登録日: 2008-08-08, 公開日: 2008-08-26, 最終更新日: 2024-02-21)

主引用文献

Babic, A.C.,Little, E.J.,Manohar, V.M.,Bitinaite, J.,Horton, N.C.
DNA distortion and specificity in a sequence-specific endonuclease.
J.Mol.Biol., 383:186-204, 2008

PubMed Abstract: Five new structures of the Q138F HincII enzyme bound to a total of three different DNA sequences and three different metal ions (Ca(2+), Mg(2+), and Mn(2+)) are presented. While previous structures were produced from soaking Ca(2+) into preformed Q138F HincII/DNA crystals, the new structures are derived from cocrystallization with Ca(2+), Mg(2+), or Mn(2+). The Mn(2)(+)-bound structure provides the first view of a product complex of Q138F HincII with cleaved DNA. Binding studies and a crystal structure show how Ca(2+) allows trapping of a Q138F HincII complex with noncognate DNA in a catalytically incompetent conformation. Many Q138F HincII/DNA structures show asymmetry, despite the binding of a symmetric substrate by a symmetric enzyme. The various complexes are fit into a model describing the different conformations of the DNA-bound enzyme and show how DNA conformational energetics determine DNA-cleavage rates by the Q138F HincII enzyme.

PubMed: 18762194
DOI: 10.1016/j.jmb.2008.08.032

実験手法

X-RAY DIFFRACTION (2.73 Å)