3E3D

Structure of hen egg white lysozyme with the magic triangle I3C

3E3D の概要

• エントリーDOI: 10.2210/pdb3e3d/pdb
• 関連するPDBエントリー: 3E3S 3E3T
• 分子名称: Lysozyme C, 5-amino-2,4,6-triiodobenzene-1,3-dicarboxylic acid, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, ... (4 entities in total)
• 機能のキーワード: phasing tool, 5-amino-2, 4, 6-triiodoisophthalic acid, i3c, magic triangle, allergen, antimicrobial, bacteriolytic enzyme, glycosidase, hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16804.81

構造登録者

Beck, T.,Gruene, T.,Sheldrick, G.M. (登録日: 2008-08-07, 公開日: 2008-10-28, 最終更新日: 2024-10-09)

主引用文献

Beck, T.,Krasauskas, A.,Gruene, T.,Sheldrick, G.M.
A magic triangle for experimental phasing of macromolecules
Acta Crystallogr.,Sect.D, 64:1179-1182, 2008

PubMed Abstract: Obtaining phase information for the solution of macromolecular structures is still one of the bottlenecks in X-ray crystallography. 5-Amino-2,4,6-triiodoisophthalic acid (I3C), in which three covalently bound iodines form an equilateral triangle, was incorporated into proteins in order to obtain phases by single-wavelength anomalous dispersion (SAD). An improved binding capability compared with simple heavy-metal ions, ready availability, improved recognition of potential heavy-atom sites and low toxicity make I3C particularly suitable for experimental phasing.

PubMed: 19020357
DOI: 10.1107/S0907444908030266

実験手法

X-RAY DIFFRACTION (1.55 Å)