3DSS

Crystal structure of RabGGTase(DELTA LRR; DELTA IG)

3DSS の概要

• エントリーDOI: 10.2210/pdb3dss/pdb
• 関連するPDBエントリー: 3C72 3DST 3DSU 3DSV 3DSW 3DSX
• 分子名称: Geranylgeranyl transferase type-2 subunit alpha, Geranylgeranyl transferase type-2 subunit beta, ZINC ION, ... (5 entities in total)
• 機能のキーワード: protein prenylation, metal-binding, prenyltransferase, transferase, zinc, phosphoprotein
• 由来する生物種: Rattus norvegicus (Rat); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 75479.32

構造登録者

Guo, Z.,Yu, S.,Goody, R.S.,Alexandrov, K.,Blankenfeldt, W. (登録日: 2008-07-14, 公開日: 2008-09-09, 最終更新日: 2023-11-01)

主引用文献

Guo, Z.,Wu, Y.-W.,Das, D.,Delon, C.,Cramer, J.,Yu, S.,Thuns, S.,Lupilova, N.,Waldmann, H.,Brunsveld, L.,Goody, R.S.,Alexandrov, K.,Blankenfeldt, W.
Structures of RabGGTase-substrate/product complexes provide insights into the evolution of protein prenylation
Embo J., 27:2444-2456, 2008

PubMed Abstract: Post-translational isoprenylation of proteins is carried out by three related enzymes: farnesyltransferase, geranylgeranyl transferase-I, and Rab geranylgeranyl transferase (RabGGTase). Despite the fact that the last one is responsible for the largest number of individual protein prenylation events in the cell, no structural information is available on its interaction with substrates and products. Here, we present structural and biophysical analyses of RabGGTase in complex with phosphoisoprenoids as well as with the prenylated peptides that mimic the C terminus of Rab7 GTPase. The data demonstrate that, unlike other protein prenyl transferases, both RabGGTase and its substrate RabGTPases completely 'outsource' their specificity for each other to an accessory subunit, the Rab escort protein (REP). REP mediates the placement of the C terminus of RabGTPase into the active site of RabGGTase through a series protein-protein interactions of decreasing strength and selectivity. This arrangement enables RabGGTase to prenylate any cysteine-containing sequence. On the basis of our structural and thermodynamic data, we propose that RabGGTase has evolved from a GGTase-I-like molecule that 'learned' to interact with a recycling factor (GDI) that, in turn, eventually gave rise to REP.

PubMed: 18756270
DOI: 10.1038/emboj.2008.164

実験手法

X-RAY DIFFRACTION (1.8 Å)