3DS7

Structure of an RNA-2'-deoxyguanosine complex

3DS7 の概要

• エントリーDOI: 10.2210/pdb3ds7/pdb
• 分子名称: 67-MER, ACETATE ION, COBALT HEXAMMINE(III), ... (5 entities in total)
• 機能のキーワード: rna, rna-ligand complex, riboswitch
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 45510.59

構造登録者

Edwards, A.L.,Batey, R.T. (登録日: 2008-07-11, 公開日: 2009-02-17, 最終更新日: 2023-08-30)

主引用文献

Edwards, A.L.,Batey, R.T.
A structural basis for the recognition of 2'-deoxyguanosine by the purine riboswitch.
J.Mol.Biol., 385:938-948, 2009

PubMed Abstract: Riboswitches are noncoding RNA elements that are commonly found in the 5'-untranslated region of bacterial mRNA. Binding of a small-molecule metabolite to the riboswitch aptamer domain guides the folding of the downstream sequence into one of two mutually exclusive secondary structures that directs gene expression. The purine riboswitch family, which regulates aspects of purine biosynthesis and transport, contains three distinct classes that specifically recognize guanine/hypoxanthine, adenine, or 2'-deoxyguanosine (dG). Structural analysis of the guanine and adenine classes revealed a binding pocket that almost completely buries the nucleobase within the core of the folded RNA. Thus, it is somewhat surprising that this family of RNA elements also recognizes dG. We have used a combination of structural and biochemical techniques to understand how the guanine riboswitch could be converted into a dG binder and the structural basis for dG recognition. These studies reveal that a limited number of sequence changes to a guanine-sensing RNA are required to cause a specificity switch from guanine to 2'-deoxyguanosine, and to impart an altered structure for accommodating the additional deoxyribose sugar moiety.

PubMed: 19007790
DOI: 10.1016/j.jmb.2008.10.074

実験手法

X-RAY DIFFRACTION (1.85 Å)